BMRB: 6269

1H, 13C, and 15N Chemical Shift Assignments for chPrP(128-242)

Authors

Lysek, D.A., Calzolai, L., Wuthrich, K.

Assembly

chPrP (128-242)

Entity

1. chPrP (128-242) (polymer, Thiol state: all disulfide bound), 117 monomers, 13325.75 Da Detail

GSVVGGLGGY AMGRVMSGMN YHFDRPDEYR WWSENSARYP NRVYYRDYSS PVPQDVFVAD CFNITVTEYS IGPAAKKNTS EAVAAANQTE VEMENKVVTK VIREMCVQQY REYRLAS

Formula weight

13325.75 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS106:SG

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.6 %, Completeness (bb): 84.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.6 % (1188 of 1341)	98.0 % (681 of 695)	73.7 % (384 of 521)	98.4 % (123 of 125)
Backbone	84.7 % (586 of 692)	99.2 % (235 of 237)	70.0 % (240 of 343)	99.1 % (111 of 112)
Sidechain	93.4 % (708 of 758)	97.4 % (446 of 458)	87.1 % (250 of 287)	92.3 % (12 of 13)
Aromatic	75.4 % (104 of 138)	92.8 % (64 of 69)	56.7 % (38 of 67)	100.0 % (2 of 2)
Methyl	99.1 % (105 of 106)	100.0 % (53 of 53)	98.1 % (52 of 53)

1. chicken prion protein

GSVVGGLGGY AMGRVMSGMN YHFDRPDEYR WWSENSARYP NRVYYRDYSS PVPQDVFVAD CFNITVTEYS IGPAAKKNTS EAVAAANQTE VEMENKVVTK VIREMCVQQY REYRLAS

Show sample condition

Sample

Temperature 293 (±1) K, pH 4.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	chicken prion protein	[U-13C; U-15N]		1.0 mM
2	sodium acetate			1.0 mM

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LACS Plot; CA

Referencing offset: 0.47 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.47 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1U3M, Strand ID: A Detail

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Release date

2004-07-21

Citation 1

NMR assignment of the chicken prion protein fragments chPrP(128-242) and chPrP(25-242)
Lysek, D.A., Calzolai, L., Wuthrich, K.
J. Biomol. NMR (2004), 30, 97-97, PubMed 15459548 , DOI 10.1023/B:JNMR.0000043504.61065.f4 , Abstract

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Citation 2

A prion-like protein from chicken brain copurifies with an acetylcholine receptor-inducing activity
Harris, D.A., Falls, D.L., Johnson, F.A., Fischbach, G.D.
Proc. Natl. Acad. Sci. U. S. A. (1991), 88, 7664-7668, PubMed 1715573 , DOI: , Abstract

Citation 3

NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231)
Riek, R., Hornemann, S., Wider, G., Glockshuber, R., Wuthrich, K.
FEBS Lett. (1997), 413, 282-288, PubMed 9280298 , DOI: , Abstract

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Related entities 1. chPrP (128-242), : 1 : 154 entities Detail

Experiments performed 4 experiments Detail