BMRB: 6282

NMR assignment of the turtle prion protein fragments tPrP(121-225)

Authors

Calzolai, L., Lysek, D., Wuthrich, K.

Assembly

turtle PrP monomer

Entity

1. turtle PrP monomer (polymer, Thiol state: all disulfide bound), 108 monomers, 12599.90 Da Detail

GSVVGGLGGY ALGSAMSGMR MNFDRPEERQ WWNENSNRYP NQVYYKEYND RSVPEGRFVR DCVNITVTEY KIDPNENQNV TQVEVRVMKQ VIQEMCMQQY QQYQLASG

Formula weight

12599.9 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS62:SG	1:CYS96:SG

Source organism

Trachemys scripta

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 83.3 %, Completeness (bb): 84.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.3 % (1064 of 1278)	93.3 % (629 of 674)	67.7 % (323 of 477)	88.2 % (112 of 127)
Backbone	84.2 % (539 of 640)	97.7 % (216 of 221)	70.5 % (222 of 315)	97.1 % (101 of 104)
Sidechain	84.5 % (623 of 737)	91.2 % (413 of 453)	76.2 % (199 of 261)	47.8 % (11 of 23)
Aromatic	53.7 % (58 of 108)	100.0 % (54 of 54)	3.8 % (2 of 52)	100.0 % (2 of 2)
Methyl	92.9 % (78 of 84)	92.9 % (39 of 42)	92.9 % (39 of 42)

1. prion protein

GSVVGGLGGY ALGSAMSGMR MNFDRPEERQ WWNENSNRYP NQVYYKEYND RSVPEGRFVR DCVNITVTEY KIDPNENQNV TQVEVRVMKQ VIQEMCMQQY QQYQLASG

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	prion protein	[U-13C; U-15N]		0.7 mM

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LACS Plot; CA

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1U5L, Strand ID: A Detail

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Release date

2004-10-17

Citation

NMR assignment of the chicken prion protein fragments chPrP(128-242) and chPrP(25-242)
Lysek, D.A., Calzolai, L., Wuthrich, K.
J. Biomol. NMR (2004), 30, 97-97, PubMed 15459548 , DOI 10.1023/B:JNMR.0000043504.61065.f4 , Abstract

Related entities 1. turtle PrP monomer, : 1 : 161 entities Detail