BMRB: 6285

15N, 13C, 1H NMR assignment of a 14kDa construct of the focal adhesion protein Talin

Authors

Fillingham, I.J., Critchley, D.R., Roberts, G.C., Barsukov, I.G.

Assembly

talin 755-889

Entity

1. talin 755-889 (polymer, Thiol state: not present), 139 monomers, 14380.78 Da Detail

GSHMQAATED GQLLRGVGAA ATAVTQALNE LLQHVKAHAT GAGPAGRYDQ ATDTILTVTE NIFSSMGDAG EMVRQARILA QATSDLVNAI KADAEGESDL ENSRKLLSAA KILADATAKM VEAAKGAAAH PDSEEQQQR

Formula weight

14380.78 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry, tortion angle dynamics, simmulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 92.8 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.8 % (1350 of 1454)	92.6 % (688 of 743)	93.2 % (522 of 560)	92.7 % (140 of 151)
Backbone	96.3 % (799 of 830)	95.8 % (275 of 287)	96.8 % (393 of 406)	95.6 % (131 of 137)
Sidechain	89.9 % (676 of 752)	90.6 % (413 of 456)	90.1 % (254 of 282)	64.3 % (9 of 14)
Aromatic	26.5 % (9 of 34)	52.9 % (9 of 17)	0.0 % (0 of 17)
Methyl	100.0 % (174 of 174)	100.0 % (87 of 87)	100.0 % (87 of 87)

1. talin 755-889

GSHMQAATED GQLLRGVGAA ATAVTQALNE LLQHVKAHAT GAGPAGRYDQ ATDTILTVTE NIFSSMGDAG EMVRQARILA QATSDLVNAI KADAEGESDL ENSRKLLSAA KILADATAKM VEAAKGAAAH PDSEEQQQR

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	talin 755-889	[U-95% 13C; U-98% 15N]		1 mM
2	phosphate buffer			20 mM
3	sodium chloride			50 mM

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.36 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1U89, Strand ID: A Detail

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Release date

2007-03-21

Citation

A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head
Fillingham, I.J., Gingras, A.R., Papagrigoriou, E., Patel, B., Emsley, J., Critchley, D.R., Roberts, G.C., Barsukov, I.L.
Structure (2005), 13, 65-74, PubMed 15642262 , DOI 10.1016/j.str.2004.11.006 , Abstract

Related entities 1. talin 755-889, : 1 : 5 : 12 entities Detail

Interaction partners 1. talin 755-889, : 31 interactors Detail

Experiments performed 1 experiments Detail