BMRBj - BMREntryMaster

Found 1 Document (1.777 seconds)

BMRB: 6295

1TVC

NMR Structure of the Flavin Domain from Soluble Methane Monooxygenase Reductase from Methylococcus capsulatus (Bath)

Authors

Chatwood, L.L., Mueller, J., Gross, J.D., Wagner, G., Lippard, S.J.

Assembly

flavin domain of methane monooxygenase reductase

Entity

1. flavin domain of methane monooxygenase reductase (polymer, Thiol state: all free), 252 monomers, 28528.74 Da Detail

MCRISFGEVG SFEAEVVGLN WVSSNTVQFL LQKRPDECGN RGVKFEPGQF MDLTIPGTDV SRSYSPANLP NPEGRLEFLI RVLPEGRFSD YLRNDARVGQ VLSVKGPLGV FGLKERGMAP RYFVAGGTGL APVVSMVRQM QEWTAPNETR IYFGVNTEPE LFYIDELKSL ERSMRNLTVK ACVWHPSGDW EGEQGSPIDA LREDLESSDA NPDIYLCGPP GMIDAACELV RSRGIPGEQV FFEKFLPSGA AX

Formula weight

28528.74 Da

Source organism

Methylococcus capsulatus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.6 %, Completeness: 82.0 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.0 % (2364 of 2883)	77.5 % (1165 of 1504)	85.0 % (956 of 1125)	95.7 % (243 of 254)
Backbone	95.6 % (1404 of 1468)	94.9 % (483 of 509)	95.2 % (692 of 727)	98.7 % (229 of 232)
Sidechain	71.9 % (1179 of 1640)	68.5 % (682 of 995)	77.5 % (483 of 623)	63.6 % (14 of 22)
Aromatic	25.0 % (60 of 240)	40.0 % (48 of 120)	7.8 % (9 of 116)	75.0 % (3 of 4)
Methyl	98.8 % (249 of 252)	99.2 % (125 of 126)	98.4 % (124 of 126)

1. Flavin domain of methane monooxygenase reductase

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	Flavin domain of methane monooxygenase reductase	[U-13C; U-15N; U-2H]	0.6 ~ 1.0 mM
2	phosphate buffer		50 mM
3	DTT		1 mM
4	NaN3		0.1 %
5	Pefabloc protease inhibitor		2 mM
6	NADH		3 mM
7	Na2S2O4		30 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1TVC, Strand ID: A Detail

Release date

2004-09-09

Citation

NMR structure of the flavin domain from soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath)
Chatwood, L.L., Mueller, J., Gross, J.D., Wagner, G., Lippard, S.J.
Biochemistry (2004), 43, 11983-11991, PubMed 15379538 , DOI 10.1021/bi049066n , Abstract

Related entities 1. flavin domain of methane monooxygenase reductase, : 1 : 1 : 86 entities Detail

Experiments performed 13 experiments Detail

1 2D NOESY