BMRB: 6298

NMR STRUCTURE OF HUMAN HISTONE CHAPERONE, ASF1A

Authors

MOUSSON, F., LAUTRETTE, A., THURET, J.Y., AGEZ, M., AMIGUES, B., BECKER, E., COURBEYRETTE, R., NEUMANN, J.M., GUEROIS, R., MANN, C., OCHSENBEIN, F.

Assembly

ASF1 anti-silencing function 1 homolog A

Entity

1. ASF1 anti-silencing function 1 homolog A (polymer, Thiol state: all free), 156 monomers, 17786.74 Da Detail

MAKVQVNNVV VLDNPSPFYN PFQFEITFEC IEDLSEDLEW KIIYVGSAES EEYDQVLDSV LVGPVPAGRH MFVFQADAPN PGLIPDADAV GVTVVLITCT YRGQEFIRVG YYVNNEYTET ELRENPPVKP DFSKLQRNIL ASNPRVTRFH INWEDN

Formula weight

17786.74 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.8 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.8 % (1744 of 1839)	95.6 % (909 of 951)	92.8 % (672 of 724)	99.4 % (163 of 164)
Backbone	98.6 % (899 of 912)	99.7 % (306 of 307)	97.6 % (450 of 461)	99.3 % (143 of 144)
Sidechain	92.2 % (992 of 1076)	93.6 % (603 of 644)	89.6 % (369 of 412)	100.0 % (20 of 20)
Aromatic	60.7 % (108 of 178)	65.2 % (58 of 89)	55.2 % (48 of 87)	100.0 % (2 of 2)
Methyl	100.0 % (182 of 182)	100.0 % (91 of 91)	100.0 % (91 of 91)

1. ASF1 anti-silencing function 1 homolog A

MAKVQVNNVV VLDNPSPFYN PFQFEITFEC IEDLSEDLEW KIIYVGSAES EEYDQVLDSV LVGPVPAGRH MFVFQADAPN PGLIPDADAV GVTVVLITCT YRGQEFIRVG YYVNNEYTET ELRENPPVKP DFSKLQRNIL ASNPRVTRFH INWEDN

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	ASF1 anti-silencing function 1 homolog A	[U-15N; U-13C]		1 mM
2	tris-D11			20 mM
3	EDTA			1 mM
4	DSS			0.1 mM
5	NaN3			0.1 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	na	0.0 ppm	null	null	1.0
15N	DSS	na	0.0 ppm	null	null	0.1013291
13C	DSS	na	0.0 ppm	null	null	0.2514495

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	na	0.0 ppm	null	null	1.0
15N	DSS	na	0.0 ppm	null	null	0.1013291
13C	DSS	na	0.0 ppm	null	null	0.2514495

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 7 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	na	0.0 ppm	null	null	1.0
15N	DSS	na	0.0 ppm	null	null	0.1013291
13C	DSS	na	0.0 ppm	null	null	0.2514495

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LACS Plot; CO

Referencing offset: 0.47 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	na	0.0 ppm	null	null	1.0
15N	DSS	na	0.0 ppm	null	null	0.1013291
13C	DSS	na	0.0 ppm	null	null	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1TEY, Strand ID: A Detail

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Release date

2005-11-06

Citation 1

1H, 13C and 15N resonance assignments of the conserved core of hAsf1 A
Mousson, F., Couprie, J., Thuret, J., Neumann, J., Mann, C., Ochsenbein, F.
J. Biomol. NMR (2004), 29, 413-414, PubMed 15213445 , DOI 10.1023/B:JNMR.0000032497.24661.44 , Abstract

Show more 1 citaion

Citation 2

Structural basis for the interaction of Asf1 with histone H3 and its functional implications
MOUSSON, F., LAUTRETTE, A., THURET, J.Y., AGEZ, M., COURBEYRETTE, R., AMIGUES, B., BECKER, E., NEUMANN, J.M., GUEROIS, R., MANN, C., OCHSENBEIN, F.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 5975-5980, PubMed 15840725 , DOI 10.1073/pnas.0500149102 , Abstract

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Related entities 1. ASF1 anti-silencing function 1 homolog A, : 1 : 16 : 1 : 6 : 85 entities Detail

Interaction partners 1. ASF1 anti-silencing function 1 homolog A, : 40 interactors Detail

Experiments performed 3 experiments Detail