BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.4 % (804 of 880)	90.1 % (411 of 456)	91.6 % (316 of 345)	97.5 % (77 of 79)
Backbone	95.6 % (455 of 476)	95.8 % (158 of 165)	94.9 % (225 of 237)	97.3 % (72 of 74)
Sidechain	88.3 % (421 of 477)	86.9 % (253 of 291)	90.1 % (163 of 181)	100.0 % (5 of 5)
Aromatic	46.7 % (14 of 30)	80.0 % (12 of 15)	7.1 % (1 of 14)	100.0 % (1 of 1)
Methyl	95.7 % (88 of 92)	97.8 % (45 of 46)	93.5 % (43 of 46)

1. Kalirin

GPLGSPGIPG STLSGGCELT VVLQDFSAAH SSELSIQVGQ TVELLERPSE RPGWCLVRTT ERSPPQEGLV PSSTLCISHS RS

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.39 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.39 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.37 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1U3O, Strand ID: A Detail

Release date

2004-08-25

Citation

Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions
Schiller, M.R., Chakrabarti, K., King, G.F., Schiller, N.I., Eipper, B.A., Maciejewski, M.W.
J. Biol. Chem. (2006), 281, 18774-18786, PubMed 16644733 , DOI 10.1074/jbc.M512482200 , Abstract

Related entities 1. Kalirin, : 1 : 37 entities Detail

Interaction partners 1. Kalirin, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 9 experiments Detail

1 HNHA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

2 HNHB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

3 3D HN(CO)CACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

4 3D C(CO)NH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

5 3D 15N NOESY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

6 3D 13C HSQC-NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

7 3D HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

8 HC(CO)NH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

9 HCCH-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	Kalirin	[U-15N]	0.4 mM
2	HEPES		50 mM
3	NaCl		150 mM
4	dithiotheitol		1 mM
5	H2O		92.5 %
6	D2O		7.5 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
7	Kalirin	[U-13C; U-15N]	1 mM
8	HEPES		50 mM
9	NaCl		150 mM
10	dithiotheitol		1 mM
11	H2O		92.5 %
12	D2O		7.5 %

nullKeywords SH3, cis-Proline

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Found 1 Document (1.779 seconds)

BMRB: 6300

Sample

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Kalirin, : 1 : 37 entities Detail

Interaction partners 1. Kalirin, : 3 interactors Detail

Experiments performed 9 experiments Detail

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