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Solution structure of rat Kalirin N-terminal SH3 domain
Authors
Schiller, M.R., Chakrabarti, K., King, G.F., Schiller, N.I., Eipper, B.A., Maciejewski, M.W.
Assembly
Kalirin
Entity
1. Kalirin (polymer, Thiol state: all free), 82 monomers, 8571.516 Da Detail

GPLGSPGIPG STLSGGCELT VVLQDFSAAH SSELSIQVGQ TVELLERPSE RPGWCLVRTT ERSPPQEGLV PSSTLCISHS RS


Formula weight
8571.516 Da
Source organism
Rattus norvegicus
Exptl. method
NMR
Refine. method
Torsion angle dynamics and simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 91.4 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.4 % (804 of 880)90.1 % (411 of 456)91.6 % (316 of 345)97.5 % (77 of 79)
Backbone95.6 % (455 of 476)95.8 % (158 of 165)94.9 % (225 of 237)97.3 % (72 of 74)
Sidechain88.3 % (421 of 477)86.9 % (253 of 291)90.1 % (163 of 181)100.0 % (5 of 5)
Aromatic46.7 % (14 of 30)80.0 % (12 of 15) 7.1 % (1 of 14)100.0 % (1 of 1)
Methyl95.7 % (88 of 92)97.8 % (45 of 46)93.5 % (43 of 46)

1. Kalirin

GPLGSPGIPG STLSGGCELT VVLQDFSAAH SSELSIQVGQ TVELLERPSE RPGWCLVRTT ERSPPQEGLV PSSTLCISHS RS

Sample

Pressure 1 atm, Temperature 298 K, pH 6.4


#NameIsotope labelingTypeConcentration
1Kalirin[U-15N]0.4 mM
2HEPES50 mM
3NaCl150 mM
4dithiotheitol1 mM
5H2O92.5 %
6D2O7.5 %

LACS Plot; CA
Referencing offset: -0.39 ppm, Outliers: 5 Detail
LACS Plot; CB
Referencing offset: -0.39 ppm, Outliers: 5 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.37 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1U3O, Strand ID: A Detail


Release date
2004-08-25
Citation
Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions
Schiller, M.R., Chakrabarti, K., King, G.F., Schiller, N.I., Eipper, B.A., Maciejewski, M.W.
J. Biol. Chem. (2006), 281, 18774-18786, PubMed 16644733 , DOI 10.1074/jbc.M512482200 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: P97924 released on 1998-07-15
    Title KALRN_RAT Entity Kalirin
Related entities 1. Kalirin, : 1 : 37 entities Detail
Interaction partners 1. Kalirin, : 3 interactors Detail
Experiments performed 9 experiments Detail
nullKeywords SH3, cis-Proline