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BMRB: 6301


6301
1U2U
NMR solution structure of a designed heterodimeric leucine zipper
Authors
Marti, D.N., Bosshard, H.R.
Assembly
DESIGNED HETERODIMERIC LEUCINE ZIPPER
Entity
1. LEUCINE ZIPPER CHAIN A (polymer, Thiol state: not present), 32 monomers, 3478.700 Da Detail

XEVAQLEKEV AQLEAENYQL EQEVAQLEHE GX


2. LEUCINE ZIPPER CHAIN B (polymer, Thiol state: not present), 32 monomers, 3498.156 Da Detail

XEVQALKKRV QALKARNYAL KQKVQALRHK GX


Total weight
6976.856 Da
Max. entity weight
3498.156 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Refine. method
DISTANCE GEOMETRY/ REGULARIZATION, SIMULATED ANNEALING, MOLECULAR DYNAMICS SIMULATION IN VACUO
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 48.4 %, Completeness: 48.4 %, Completeness (bb): 51.1 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All48.4 % (222 of 459)47.9 % (186 of 388)50.7 % (36 of 71)
Backbone51.1 % (93 of 182)51.6 % (63 of 122)50.0 % (30 of 60)
Sidechain46.6 % (129 of 277)46.2 % (123 of 266)54.5 % (6 of 11)
Aromatic50.0 % (6 of 12)50.0 % (6 of 12)
Methyl48.6 % (18 of 37)48.6 % (18 of 37)

1. LEUCINE ZIPPER CHAIN A

XEVAQLEKEV AQLEAENYQL EQEVAQLEHE GX

2. LEUCINE ZIPPER CHAIN B

XEVQALKKRV QALKARNYAL KQKVQALRHK GX

Show sample condition
Sample

Pressure 1 atm, Temperature 310.00 (±0.5) K, pH 5.7 (±0.03)


#NameIsotope labelingTypeConcentration
1LEUCINE ZIPPER CHAIN A2.6 mM
2LEUCINE ZIPPER CHAIN B2.6 mM
3NaCl10 mM
4H2O90 %
5D2O10 %

Chem. Shift Complete2
Sequence coverage: 48.4 %, Completeness: 51.0 %, Completeness (bb): 50.8 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All51.0 % (468 of 918)51.3 % (398 of 776)49.3 % (70 of 142)
Backbone50.8 % (185 of 364)51.6 % (126 of 244)49.2 % (59 of 120)
Sidechain51.1 % (283 of 554)51.1 % (272 of 532)50.0 % (11 of 22)
Aromatic50.0 % (12 of 24)50.0 % (12 of 24)
Methyl50.0 % (37 of 74)50.0 % (37 of 74)

1. LEUCINE ZIPPER CHAIN A

XEVAQLEKEV AQLEAENYQL EQEVAQLEHE GX

2. LEUCINE ZIPPER CHAIN B

XEVQALKKRV QALKARNYAL KQKVQALRHK GX

Show sample condition
Sample

Pressure 1 atm, Temperature 310.00 (±0.5) K, pH 5.7 (±0.03)


#NameIsotope labelingTypeConcentration
1LEUCINE ZIPPER CHAIN A2.6 mM
2LEUCINE ZIPPER CHAIN B2.6 mM
3NaCl10 mM
4H2O90 %
5D2O10 %

Protein Blocks Logo
Calculated from 27 models in PDB: 1U2U, Strand ID: A, B Detail

Release date
2008-07-15
Citation
Inverse electrostatic effect: electrostatic repulsion in the unfolded state stabilizes a leucine zipper
Marti, D.N., Bosshard, H.R.
Biochemistry (2004), 43, 12436-12447, PubMed 15449933 , DOI 10.1021/bi048771t ,
Related entities 1. LEUCINE ZIPPER CHAIN A, : 28 entities Detail
More details for 28 related entities
Related entities 2. LEUCINE ZIPPER CHAIN B, : 5 entities Detail
More details for 5 related entities
Experiments performed 5 experiments Detail
nullKeywords COILED COIL, ELECTROSTATIC INTERACTIONS, INTER-HELICAL ION PAIRING, LEUCINE ZIPPER