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Found 1 Document (5.485 seconds)

BMRB: 6311

2AFJ

Sequence-specific backbone 1H, 13C and 15N assignments of the 25 kDa SPRY domain-containing SOCS box protein 2 (SSB-2)

Authors

Yao, S., Masters, S.L., Zhang, J., Palmer, K.R., Babon, J.J., Hilton, D.J., Nicola, N.A., Nicholson, S.E., Norton, R.S.

Assembly

Spry domain-containing SOCS box protein 2

Entity

1. Spry domain-containing SOCS box protein 2 (polymer, Thiol state: all free), 226 monomers, 24859.55 Da Detail

GSSARQSTPT SQALYSDFSP PEGLEELLSA PPPDLVAQRH HGWNPKDCSE NIDVKEGGLC FERRPVAQST DGVRGKRGYS RGLHAWEISW PLEQRGTHAV VGVATALAPL QADHYAALLG SNSESWGWDI GRGKLYHQSK GLEAPQYPAG PQGEQLVVPE RLLVVLDMEE GTLGYSIGGT YLGPAFRGLK GRTLYPSVSA VWGQCQVRIR YMGERRVEET RRIHRD

Formula weight

24859.55 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 42.2 %, Completeness (bb): 75.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.2 % (1079 of 2554)	20.9 % (279 of 1333)	61.3 % (607 of 990)	83.5 % (193 of 231)
Backbone	75.2 % (996 of 1324)	46.4 % (215 of 463)	90.3 % (588 of 651)	91.9 % (193 of 210)
Sidechain	17.8 % (255 of 1429)	7.4 % (64 of 870)	35.5 % (191 of 538)	0.0 % (0 of 21)
Aromatic	0.0 % (0 of 202)	0.0 % (0 of 101)	0.0 % (0 of 95)	0.0 % (0 of 6)
Methyl	14.7 % (33 of 224)	9.8 % (11 of 112)	19.6 % (22 of 112)

1. Spry domain-containing SOCS box protein

Show sample condition

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.24 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AFJ, Strand ID: A Detail

Release date

2005-02-07

Citation

Backbone 1H, 13C and 15N assignments of the 25 kDa SPRY domain-containing SOCS box protein 2 (SSB-2)
Yao, S., Masters, S.L., Zhang, J., Palmer, K.R., Babon, J.J., Nicola, N.A., Nicholson, S.E., Norton, R.S.
J. Biomol. NMR (2005), 31, 69-70, PubMed 15692743 , DOI 10.1007/s10858-004-6924-6 , Abstract

Related entities 1. Spry domain-containing SOCS box protein 2, : 1 : 1 : 49 entities Detail

Interaction partners 1. Spry domain-containing SOCS box protein 2, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 HNCA

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

2 HN(CO)CA

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

3 HNCO

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

4 HN(CA)CO

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

5 HNCACB

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

6 CBCA(CO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

7 1H-15N NOESY

Instrument

#1 Model Bruker AVANCE (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Spry domain-containing SOCS box protein			0.60 mM

Sample #2

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Spry domain-containing SOCS box protein	[U-15N]		0.40 mM

Sample #3

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Spry domain-containing SOCS box protein	[U-13C; U-15N]		0.6 mM

Sample #4

Temperature 295 (±0.2) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Spry domain-containing SOCS box protein	[U-13C; U-15N; U-70% 2H]		1.0 mM

nullKeywords NMR assignment, SOCS protein, SOX box, SPRY domain

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Found 1 Document (5.485 seconds)

BMRB: 6311

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Chemical shift reference

Chemical shift reference

Related entities 1. Spry domain-containing SOCS box protein 2, : 1 : 1 : 49 entities Detail

Interaction partners 1. Spry domain-containing SOCS box protein 2, : 2 interactors Detail

Experiments performed 7 experiments Detail

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