BMRB: 6315

1H, 13C and 15N chemical shift assignments for TcUBP1 (GQ) (backbone + side chains)

Authors

Volpon, L., D'orso, I., Gehring, K.

Assembly

TcUBP1

Entity

1. TcUBP1 (polymer, Thiol state: all free), 139 monomers, 15413.24 Da Detail

MSQIPLVSQY DPYGQTAQLQ QLQQQQQQHI PPTQMNPEPD VLRNLMVNYI PTTVDEVQLR QLFERYGPIE SVKIVCDRET RQSRGYGFVK FQSGSSAQQA IAGLNGFNIL NKRLKVALAA SGHQRPGIAG AVGDGNGYL

Formula weight

15413.24 Da

Source organism

Trypanosoma cruzi

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 78.4 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.4 % (1275 of 1627)	75.8 % (649 of 856)	81.3 % (499 of 614)	80.9 % (127 of 157)
Backbone	94.9 % (774 of 816)	95.4 % (269 of 282)	94.1 % (380 of 404)	96.2 % (125 of 130)
Sidechain	66.4 % (622 of 937)	66.2 % (380 of 574)	71.4 % (240 of 336)	7.4 % (2 of 27)
Aromatic	20.8 % (20 of 96)	41.7 % (20 of 48)	0.0 % (0 of 48)
Methyl	85.1 % (126 of 148)	94.6 % (70 of 74)	75.7 % (56 of 74)

1. TcUBP1

MSQIPLVSQY DPYGQTAQLQ QLQQQQQQHI PPTQMNPEPD VLRNLMVNYI PTTVDEVQLR QLFERYGPIE SVKIVCDRET RQSRGYGFVK FQSGSSAQQA IAGLNGFNIL NKRLKVALAA SGHQRPGIAG AVGDGNGYL

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 7.2 (±0.1), Details In M9 minimal medium containing 0.5 g of 15N-NH4Cl and/or 2 g of 13C6-Glucose per liter of media + 100 mg/ml of ampicilin.

#	Name	Isotope labeling	Type	Concentration
1	TcUBP1	[U-13C; U-15N]		1.5 mM
2	Tris			50 mM
3	NaCl			50 mM
4	DTT			1 mM
5	NaN3			1 mM

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LACS Plot; CA

Referencing offset: 0.39 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.772925 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.39 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.772925 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.772925 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.35 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.772925 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1U6F, Strand ID: A Detail

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Release date

2005-05-02

Citation

NMR structural study of TcUBP1, a single RRM domain protein from Trypanosoma cruzi: contribution of a beta hairpin to RNA binding
Volpon, L., D'orso, I., Young, C.R., Frasch, A.C., Gehring, K.
Biochemistry (2005), 44, 3708-3717, PubMed 15751947 , DOI 10.1021/bi047450e , Abstract

Related entities 1. TcUBP1, : 1 : 3 entities Detail

Experiments performed 1 experiments Detail