Chemical Shift assignment of the blue shifted inetrmediate state of Delta25-PYP
LAFGAIQLDG DGNILQYNAA EGDITGRDPK QVIGKNFFKD VAPCTDSPEF YGKFKEGVAS GNLNTMFEYT FDYQMTPTKV KVHMKKALSG DSYWVFVKRV
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 60.8 % (717 of 1179) | 68.7 % (420 of 611) | 42.6 % (197 of 462) | 94.3 % (100 of 106) |
Backbone | 78.7 % (466 of 592) | 90.8 % (187 of 206) | 65.2 % (189 of 290) | 93.8 % (90 of 96) |
Sidechain | 49.5 % (335 of 677) | 57.5 % (233 of 405) | 35.1 % (92 of 262) | 100.0 % (10 of 10) |
Aromatic | 19.9 % (27 of 136) | 38.2 % (26 of 68) | 0.0 % (0 of 67) | 100.0 % (1 of 1) |
Methyl | 51.1 % (48 of 94) | 78.7 % (37 of 47) | 23.4 % (11 of 47) |
1. Delta25-PYP light state
LAFGAIQLDG DGNILQYNAA EGDITGRDPK QVIGKNFFKD VAPCTDSPEF YGKFKEGVAS GNLNTMFEYT FDYQMTPTKV KVHMKKALSG DSYWVFVKRVTemperature 293 (±0) K, pH 7 (±0)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Delta25-PYP light state | [U-13C; U-15N] | 1 mM | |
2 | phosphate buffer | 50 mM |
Temperature 293 (±0) K, pH 7 (±0)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Delta25-PYP light state | [U-15N] | 1 mM | |
4 | phosphate buffer | 50 mM |