BMRB: 6325

Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting

Authors

Sharpe, B.K., Liew, C.K., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.

Assembly

CREB Binding Protein (E.C.2.3.1.48)

Entity

1. CREB Binding Protein (E.C.2.3.1.48) (polymer, Thiol state: all other bound), 25 monomers, 2683.132 Da Detail

FVSTCYLPKC AAAANVAAHI THCYK

2. ZN (non-polymer), 65.409 Da

Total weight

2748.541 Da

Max. entity weight

2683.132 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS5:SG	2:ZN1:ZN
2	na	sing	1:CYS10:SG	2:ZN1:ZN
3	na	sing	1:HIS19:ND1	2:ZN1:ZN
4	na	sing	1:CYS23:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry simulated annealing molecular dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.2 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	88.2 % (120 of 136)	88.2 % (120 of 136)
Backbone	98.0 % (48 of 49)	98.0 % (48 of 49)
Sidechain	82.8 % (72 of 87)	82.8 % (72 of 87)
Aromatic	58.8 % (10 of 17)	58.8 % (10 of 17)
Methyl	93.8 % (15 of 16)	93.8 % (15 of 16)

1. CREB Binding Protein (E.C.2.3.1.48)

FVSTCYLPKC AAAANVAAHI THCYK

Show sample condition

Sample

Pressure 1 atm, Temperature 275 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	CREB Binding Protein (E.C.2.3.1.48)			0.25 mM
2	TCEP			0.000 mM
3	ZnSO4			1 mM
4	D2O			5 %
5	H2O			95 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WO7, Strand ID: A Detail

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Coupling constant

5 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 275 K, pH 7.0 Detail

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Pressure 1 atm, Temperature 275 K, pH 7.0

List #1 coupling_constants_set_1, Field strength (¹H) 600 MHz

Release date

2005-03-15

Citation

Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting
Sharpe, B.K., Liew, C.K., Kwan, A.H., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.
Structure (2005), 13, 257-266, PubMed 15698569 , DOI 10.1016/j.str.2004.12.007 , Abstract

Related entities 1. CREB Binding Protein (E.C.2.3.1.48), : 1 : 32 entities Detail

Experiments performed 3 experiments Detail