BMRB: 6326

Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting

Authors

Sharpe, B.K., Liew, C.K., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.

Assembly

CREB Binding Protein

Entity

1. CREB Binding Protein (E.C.2.3.1.48) (polymer, Thiol state: all other bound), 25 monomers, 2667.132 Da Detail

AVYYCILPKC AAAANVAAHT THCFK

2. ZN (non-polymer), 65.409 Da

Total weight

2732.541 Da

Max. entity weight

2667.132 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS5:SG	2:ZN1:ZN
2	na	sing	1:CYS10:SG	2:ZN1:ZN
3	na	sing	1:HIS19:ND1	2:ZN1:ZN
4	na	sing	1:CYS23:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.9 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.9 % (116 of 135)	85.9 % (116 of 135)
Backbone	93.9 % (46 of 49)	93.9 % (46 of 49)
Sidechain	81.4 % (70 of 86)	81.4 % (70 of 86)
Aromatic	58.8 % (10 of 17)	58.8 % (10 of 17)
Methyl	100.0 % (17 of 17)	100.0 % (17 of 17)

1. CREB Binding Protein (E.C.2.3.1.48)

AVYYCILPKC AAAANVAAHT THCFK

Show sample condition

Sample

Pressure 1 atm, Temperature 275 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	CREB Binding Protein (E.C.2.3.1.48)			0.3 mM
2	TCEP			0.5 mM
3	ZnSO4			1 mM
4	D2O			5 %
5	H2O			95 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WO6, Strand ID: A Detail

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Release date

2005-03-15

Citation

Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting
Sharpe, B.K., Liew, C.K., Kwan, A.H., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.
Structure (2005), 13, 257-266, PubMed 15698569 , DOI 10.1016/j.str.2004.12.007 , Abstract

Related entities 1. CREB Binding Protein (E.C.2.3.1.48), : 1 : 27 entities Detail

Experiments performed 3 experiments Detail