BMRB: 6327

Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting

Authors

Sharpe, B.K., Liew, C.K., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.

Assembly

CREB Binding Protein (E.C.2.3.1.48)

Entity

1. CREB Binding Protein (E.C.2.3.1.48) (polymer, Thiol state: all other bound), 25 monomers, 2600.156 Da Detail

RVIACFLKVC AAAANVAAHM THCAK

2. ZN (non-polymer), 65.409 Da

Total weight

2665.565 Da

Max. entity weight

2600.156 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS5:SG	2:ZN1:ZN
2	na	sing	1:CYS10:SG	2:ZN1:ZN
3	na	sing	1:HIS19:ND1	2:ZN1:ZN
4	na	sing	1:CYS23:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 78.0 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	78.0 % (103 of 132)	78.0 % (103 of 132)
Backbone	90.0 % (45 of 50)	90.0 % (45 of 50)
Sidechain	70.7 % (58 of 82)	70.7 % (58 of 82)
Aromatic	66.7 % (6 of 9)	66.7 % (6 of 9)
Methyl	89.5 % (17 of 19)	89.5 % (17 of 19)

1. CREB Binding Protein (E.C.2.3.1.48)

RVIACFLKVC AAAANVAAHM THCAK

Show sample condition

Sample

Pressure 1 atm, Temperature 275 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	CREB Binding Protein (E.C.2.3.1.48)			0.3 mM
2	TCEP			0.5 mM
3	ZnSO4			1 mM
4	D2O			5 %
5	H2O			95 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WO5, Strand ID: A Detail

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Release date

2005-03-15

Citation

Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting
Sharpe, B.K., Liew, C.K., Kwan, A.H., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.
Structure (2005), 13, 257-266, PubMed 15698569 , DOI 10.1016/j.str.2004.12.007 , Abstract

Related entities 1. CREB Binding Protein (E.C.2.3.1.48), : 1 : 21 entities Detail

Experiments performed 3 experiments Detail