Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS5:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS10:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS19:ND1 | 2:ZN1:ZN |
4 | na | sing | 1:CYS23:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 86.2 % (100 of 116) | 86.2 % (100 of 116) |
Backbone | 92.0 % (46 of 50) | 92.0 % (46 of 50) |
Sidechain | 81.8 % (54 of 66) | 81.8 % (54 of 66) |
Aromatic | 100.0 % (4 of 4) | 100.0 % (4 of 4) |
Methyl | 100.0 % (17 of 17) | 100.0 % (17 of 17) |
1. CREB Binding Protein (E.C.2.3.1.48)
AVSACALSKC AAAANVAAHM THCAKPressure 1 atm, Temperature 275 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CREB Binding Protein (E.C.2.3.1.48) | 0.4 mM | ||
2 | TCEP | 0.5 mM | ||
3 | ZnSO4 | 1 mM | ||
4 | D2O | 100 % |