BMRB: 6329

Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting

Authors

Sharpe, B.K., Liew, C.K., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.

Assembly

CREB Binding Protein (E.C.2.3.1.48)

Entity

1. CREB Binding Protein (E.C.2.3.1.48) (polymer, Thiol state: all other bound), 25 monomers, 2410.856 Da Detail

AVSACALPKC AAAANVAAHM THCAK

2. ZN (non-polymer), 65.409 Da

Total weight

2476.265 Da

Max. entity weight

2410.856 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS5:SG	2:ZN1:ZN
2	na	sing	1:CYS10:SG	2:ZN1:ZN
3	na	sing	1:HIS19:ND1	2:ZN1:ZN
4	na	sing	1:CYS23:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.9 %, Completeness (bb): 95.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	89.9 % (107 of 119)	89.9 % (107 of 119)
Backbone	95.9 % (47 of 49)	95.9 % (47 of 49)
Sidechain	85.7 % (60 of 70)	85.7 % (60 of 70)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (17 of 17)	100.0 % (17 of 17)

1. CREB Binding Protein (E.C.2.3.1.48)

AVSACALPKC AAAANVAAHM THCAK

Show sample condition

Sample

Pressure 1 atm, Temperature 275 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	CREB Binding Protein (E.C.2.3.1.48)			0.4 mM
2	TCEP			0.5 mM
3	ZnSO4			1 mM
4	D2O			5 %
5	H2O			95 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WO3, Strand ID: A Detail

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Release date

2005-03-15

Citation

Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting
Sharpe, B.K., Liew, C.K., Kwan, A.H., Wilce, J.A., Crossley, M., Matthews, J.M., Mackay, J.P.
Structure (2005), 13, 257-266, PubMed 15698569 , DOI 10.1016/j.str.2004.12.007 , Abstract

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. CREB Binding Protein (E.C.2.3.1.48), : 1 : 1 : 33 entities Detail

Experiments performed 4 experiments Detail