BMRBj - BMREntryMaster

Found 1 Document (6.777 seconds)

BMRB: 6336

1XSB

Assignment of human AP4A hydrolase in complex with ATP

Authors

Swarbrick, J.D.

Assembly

AP4A hydrolase with ATP

Entity

1. AP4A (polymer, Thiol state: all free), 153 monomers, 17295.61 Da Detail

GPLGSMALRA CGLIIFRRCL IPKVDNNAIE FLLLQASDGI HHWTPPKGHV EPGEDDLETA LRATQEEAGI EAGQLTIIEG FKRELNYVAR NKPKTVIYWL AEVKDYDVEI RLSHEHQAYR WLGLEEACQL AQFKEMKAAL QEGHQFLCSI EAL

2. ATP (non-polymer), 507.181 Da

Total weight

17802.791 Da

Max. entity weight

17295.61 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

CANDID

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.7 % (1726 of 1804)	96.5 % (905 of 938)	94.0 % (662 of 704)	98.1 % (159 of 162)
Backbone	98.9 % (896 of 906)	99.7 % (310 of 311)	98.2 % (440 of 448)	99.3 % (146 of 147)
Sidechain	93.4 % (971 of 1040)	94.9 % (595 of 627)	91.2 % (363 of 398)	86.7 % (13 of 15)
Aromatic	82.4 % (117 of 142)	95.8 % (68 of 71)	67.6 % (46 of 68)	100.0 % (3 of 3)
Methyl	98.4 % (183 of 186)	98.9 % (92 of 93)	97.8 % (91 of 93)

1. AP4A

GPLGSMALRA CGLIIFRRCL IPKVDNNAIE FLLLQASDGI HHWTPPKGHV EPGEDDLETA LRATQEEAGI EAGQLTIIEG FKRELNYVAR NKPKTVIYWL AEVKDYDVEI RLSHEHQAYR WLGLEEACQL AQFKEMKAAL QEGHQFLCSI EAL

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	AP4A	[U-95% 13C; U-95% 15N]		1.0 mM
2	ADENOSINE-5'-TRIPHOSPHATE	[U-95% 13C; U-95% 15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 39 models in PDB: 1XSB, Strand ID: A Detail

Release date

2005-11-13

Citation 1

1H, 13C, and 15N resonance assignments of the 17 kDa Ap4A hydrolase from Homo sapiens in the presence and absence of ATP
Swarbrick, J.D., Buyya, S., Gunawardana, D., Fletcher, J.L., Branson, K., Smith, B., Pepe, S., McLennan, A.G., Gayler, K.R., Gooley, P.R.
J. Biomol. NMR (2005), 31, 181-182, PubMed 15772762 , DOI 10.1007/s10858-004-7440-4 , Abstract

Show more 1 citaion

Citation 2

Structure and substrate-binding mechanism of human Ap4A hydrolase
Swarbrick, J.D., Buyya, S., Gunawardana, D., Gayler, K.R., McLennan, A.G., Gooley, P.R.
J. Biol. Chem. (2005), 280, 8471-8481, PubMed 15596429 , DOI 10.1074/jbc.M412318200 , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 6330 released on 2004-12-21
    Title Solution structure of human AP4A hydrolase
  Swiss-Prot: P50583 released on 1996-10-01
    Title AP4A_HUMAN Entity Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]

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Found 1 Document (6.777 seconds)

BMRB: 6336

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. AP4A, : 1 : 2 : 5 : 31 entities Detail

Interaction partners 1. AP4A, : 2 interactors Detail

Experiments performed 1 experiments Detail

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Sample