BMRB: 6337

Solution Structure of Ubiquitin like protein from Mus musculus

Authors

Singh, S., Tonelli, M., Tyler, R.C., Lee, M.S., Markley, J.L.

Assembly

AAH26994

Entity

1. AAH26994 (polymer, Thiol state: all free), 101 monomers, 11278.81 Da Detail

SAAPLCVKVE FGGGAELLFD GVKKHQVALP GQEEPWDIRN LLVWIKKNLL KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SILFISTLHG G

Formula weight

11278.81 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle, cartesian coordinate dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.0 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.0 % (1096 of 1205)	92.8 % (581 of 626)	87.5 % (412 of 471)	95.4 % (103 of 108)
Backbone	96.6 % (576 of 596)	97.6 % (202 of 207)	95.9 % (281 of 293)	96.9 % (93 of 96)
Sidechain	87.0 % (609 of 700)	90.5 % (379 of 419)	81.8 % (220 of 269)	83.3 % (10 of 12)
Aromatic	22.8 % (21 of 92)	39.1 % (18 of 46)	0.0 % (0 of 43)	100.0 % (3 of 3)
Methyl	97.1 % (132 of 136)	98.5 % (67 of 68)	95.6 % (65 of 68)

1. AAH26994

SAAPLCVKVE FGGGAELLFD GVKKHQVALP GQEEPWDIRN LLVWIKKNLL KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SILFISTLHG G

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	AAH26994	[U-15N; U-13C]		1 mM
2	Bis-Tris buffer			5 mM

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XO3, Strand ID: A Detail

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Release date

2004-10-11

Citation

Solution structure of Ubiquitin like protein from Mus musculus
Singh, S., Tonelli, M., Tyler, R.C., Lee, M.S., Markley, J.L.
J. Biol. Chem.

Related entities 1. AAH26994, : 1 : 3 : 76 entities Detail

Interaction partners 1. AAH26994, : 12 interactors Detail

Experiments performed 8 experiments Detail