BMRB: 6338

NMR solution structure of At1g77540

Authors

Tyler, R.C., Singh, S., Tonelli, M., Lee, M., Markley, J.L.

Assembly

At1g77540

Entity

1. At1g77540 (polymer, Thiol state: all free), 103 monomers, 11734.29 Da Detail

MATEPPKIVW NEGKRRFETE DHEAFIEYKM RNNGKVMDLV HTYVPSFKRG LGLASHLCVA AFEHASSHSI SIIPSCSYVS DTFLPRNPSW KPLIHSEVFK SSI

Formula weight

11734.29 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

Torsion Angle Dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 74.1 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.1 % (906 of 1222)	70.1 % (445 of 635)	75.7 % (367 of 485)	92.2 % (94 of 102)
Backbone	93.0 % (562 of 604)	93.6 % (190 of 203)	91.8 % (280 of 305)	95.8 % (92 of 96)
Sidechain	60.9 % (437 of 717)	59.0 % (255 of 432)	64.5 % (180 of 279)	33.3 % (2 of 6)
Aromatic	17.4 % (23 of 132)	30.3 % (20 of 66)	1.6 % (1 of 64)	100.0 % (2 of 2)
Methyl	76.0 % (76 of 100)	78.0 % (39 of 50)	74.0 % (37 of 50)

1. At1g77540

MATEPPKIVW NEGKRRFETE DHEAFIEYKM RNNGKVMDLV HTYVPSFKRG LGLASHLCVA AFEHASSHSI SIIPSCSYVS DTFLPRNPSW KPLIHSEVFK SSI

Show sample condition

Sample

Temperature 300 (±0.05) K, pH 6.0 (±0.1), Details 13C/15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	At1g77540	[U-13C; U-15N]		0.9 ~ 1.1 mM
2	KHPO4			10 mM

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LACS Plot; CA

Referencing offset: -0.39 ppm, Outliers: 6 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.39 ppm, Outliers: 6 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EVN, Strand ID: A Detail

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Release date

2005-12-27

Citation

Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family
Tyler, R.C., Bitto, E., Berndsen, C.E., Bingman, C.A., Singh, S., Lee, M.S., Wesenberg, G.E., Denu, J.M., Phillips, G.N., Markley, J.L.
Biochemistry (2006), 45, 14325-14336, PubMed 17128971 , DOI 10.1021/bi0612059 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. At1g77540, : 1 : 4 : 2 : 7 entities Detail

Experiments performed 7 experiments Detail