BMRB: 6339

Solution structure of AT1g01470 from Arabidopsis Thaliana

Authors

Singh, S.

Assembly

AT1g01470 monomer

Entity

1. AT1g01470 monomer (polymer, Thiol state: all free), 151 monomers, 16542.83 Da Detail

MASLLDKAKD FVADKLTAIP KPEGSVTDVD LKDVNRDSVE YLAKVSVTNP YSHSIPICEI SFTFHSAGRE IGKGKIPDPG SLKAKDMTAL DIPVVVPYSI LFNLARDVGV DWDIDYELQI GLTIDLPVVG EFTIPISSKG EIKLPTFKDF F

Formula weight

16542.83 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle and cartesian coordinate dynamics; water refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 85.3 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.3 % (1496 of 1753)	84.9 % (767 of 903)	83.3 % (587 of 705)	97.9 % (142 of 145)
Backbone	95.8 % (847 of 884)	97.0 % (291 of 300)	94.4 % (419 of 444)	97.9 % (137 of 140)
Sidechain	77.9 % (788 of 1011)	78.9 % (476 of 603)	76.2 % (307 of 403)	100.0 % (5 of 5)
Aromatic	33.3 % (44 of 132)	53.0 % (35 of 66)	12.3 % (8 of 65)	100.0 % (1 of 1)
Methyl	90.9 % (180 of 198)	93.9 % (93 of 99)	87.9 % (87 of 99)

1. Arabidopsis thaliana

MASLLDKAKD FVADKLTAIP KPEGSVTDVD LKDVNRDSVE YLAKVSVTNP YSHSIPICEI SFTFHSAGRE IGKGKIPDPG SLKAKDMTAL DIPVVVPYSI LFNLARDVGV DWDIDYELQI GLTIDLPVVG EFTIPISSKG EIKLPTFKDF F

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Arabidopsis thaliana	[U-13C; U-15N]		0.5 ~ 1.0 mM

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LACS Plot; CA

Referencing offset: -0.3 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.3 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.57 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.773 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XO8, Strand ID: A Detail

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Release date

2004-10-05

Citation

Solution structure of a late embryogenesis abundant protein (LEA14) from Arabidopsis thaliana, a cellular stress-related protein
Singh, S.
Protein Sci. (2005), 14, 2601-2609, PubMed 16155204 , DOI 10.1110/ps.051579205 , Abstract

Related entities 1. AT1g01470 monomer, : 1 : 2 : 22 entities Detail

Interaction partners 1. AT1g01470 monomer, : 1 interactors Detail

Experiments performed 8 experiments Detail