BMRB: 6341

Solution structure of At3g04780.1, an Arabidopsis ortholog of the C-terminal domain of human thioredoxin-like protein

Authors

Song, J., Tyler, R.C., Lee, M.S., Markley, J.L.

Assembly

At3g04780.1

Entity

1. At3g04780.1 (polymer, Thiol state: all free), 161 monomers, 17899.83 Da Detail

SSAESASQIP KGQVDLLDFI DWSGVECLNQ SSSHSLPNAL KQGYREDEGL NLESDADEQL LIYIPFNQVI KLHSFAIKGP EEEGPKTVKF FSNKEHMCFS NVNDFPPSDT AELTEENLKG KPVVLKYVKF QNVRSLTIFI EANQSGSEVT KVQKIALYGS T

Formula weight

17899.83 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 86.4 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.4 % (1631 of 1888)	85.7 % (843 of 984)	84.7 % (619 of 731)	97.7 % (169 of 173)
Backbone	95.8 % (910 of 950)	96.9 % (313 of 323)	94.5 % (448 of 474)	97.4 % (149 of 153)
Sidechain	79.7 % (869 of 1090)	80.2 % (530 of 661)	78.0 % (319 of 409)	100.0 % (20 of 20)
Aromatic	38.4 % (56 of 146)	45.2 % (33 of 73)	30.6 % (22 of 72)	100.0 % (1 of 1)
Methyl	86.3 % (145 of 168)	89.3 % (75 of 84)	83.3 % (70 of 84)

1. At3g04780.1

SSAESASQIP KGQVDLLDFI DWSGVECLNQ SSSHSLPNAL KQGYREDEGL NLESDADEQL LIYIPFNQVI KLHSFAIKGP EEEGPKTVKF FSNKEHMCFS NVNDFPPSDT AELTEENLKG KPVVLKYVKF QNVRSLTIFI EANQSGSEVT KVQKIALYGS T

Show sample condition

Sample

Temperature 298 K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	At3g04780.1	[U-15N; U-13C]		1 mM
2	DTT			10 mM
3	KH2PO4			50 mM

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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LACS Plot; CO

Referencing offset: 0.43 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XOY, Strand ID: A Detail

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Release date

2004-10-31

Citation

Solution structure of At3g04780.1-des15, an Arabidopsis thaliana ortholog of the C-terminal domain of human thioredoxin-like protein
Song, J., Tyler, R.C., Lee, M.S., Markley, J.L.
Protein Sci. (2005), 14, 1059-1063, PubMed 15741346 , DOI 10.1110/ps.041246805 , Abstract

Related entities 1. At3g04780.1, : 1 : 2 : 40 entities Detail

Experiments performed 9 experiments Detail