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BMRB: 6344

1XPW

1H, 13C, and 15N Chemical Shift Assignments for human protein HSPCO34

Authors

Ramelot, T.A., Montelione, G.T., Acton, T.B., Kennedy, M.A.

Assembly

HR1958

Entity

1. HR1958 (polymer, Thiol state: all free), 153 monomers, 17417.32 Da Detail

MGHHHHHHSH MRKIDLCLSS EGSEVILATS SDEKHPPENI IDGNPETFWT TTGMFPQEFI ICFHKHVRIE RLVIQSYFVQ TLKIEKSTSK EPVDFEQWIE KDLVHTEGQL QNEEIVAHGS ATYLRFIIVS AFDHFASVHS VSAEGTVVSN LSS

Formula weight

17417.32 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

XPLOR SIMULATED ANNEALING, CNS WATER REFINEMENT

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 91.3 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1632 of 1787)	91.2 % (837 of 918)	91.3 % (647 of 709)	92.5 % (148 of 160)
Backbone	92.4 % (839 of 908)	92.2 % (284 of 308)	92.3 % (417 of 452)	93.2 % (138 of 148)
Sidechain	90.5 % (928 of 1025)	90.7 % (553 of 610)	90.6 % (365 of 403)	83.3 % (10 of 12)
Aromatic	74.2 % (138 of 186)	74.2 % (69 of 93)	73.6 % (67 of 91)	100.0 % (2 of 2)
Methyl	97.6 % (164 of 168)	96.4 % (81 of 84)	98.8 % (83 of 84)

1. HR1958

MGHHHHHHSH MRKIDLCLSS EGSEVILATS SDEKHPPENI IDGNPETFWT TTGMFPQEFI ICFHKHVRIE RLVIQSYFVQ TLKIEKSTSK EPVDFEQWIE KDLVHTEGQL QNEEIVAHGS ATYLRFIIVS AFDHFASVHS VSAEGTVVSN LSS

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	HR1958	[U-15N; U-13C]	1 mM
2	Mes		20 mM
3	NaCl		100 mM
4	CaCl2		5 mM
5	DTT		10 mM
6	NaN3		0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.79 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1XPW, Strand ID: A Detail

Release date

2004-11-07

Citation

Solution NMR Structure of Human protein HSPCO34. Northeast Structural Genomics Target HR1958
Ramelot, T.A., Kennedy, M.A.

Related entities 1. HR1958, : 1 : 1 : 12 entities Detail

Interaction partners 1. HR1958, : 16 interactors Detail

More details for 16 interactors identified by 4 citations

Experiments performed 19 experiments Detail

1 Data collected on 600mHz Varian Inova spectrometer.