BMRB: 6347

Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins

Authors

Regaya, I., Beeton, C., Ferrat, G., Andreotti, N., Chandy, G.K., Darbon, H., De Waard, M., Sabatier, J.M.

Assembly

MTx-HsTx1

Entity

1. MTx-HsTx1 (polymer, Thiol state: all disulfide bound), 34 monomers, 3793.494 Da Detail

VSCTGSKDCY APCRKQTGCP YGKCMNRKCK CNRC

Formula weight

3793.494 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS24:SG
2	disulfide	sing	1:CYS9:SG	1:CYS29:SG
3	disulfide	sing	1:CYS13:SG	1:CYS31:SG
4	disulfide	sing	1:CYS19:SG	1:CYS34:SG

Source organism

Heterometrus spinifer

Exptl. method

NMR

Refine. method

distance geometry, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 72.4 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	72.4 % (147 of 203)	72.4 % (147 of 203)
Backbone	97.1 % (67 of 69)	97.1 % (67 of 69)
Sidechain	59.7 % (80 of 134)	59.7 % (80 of 134)
Aromatic	50.0 % (4 of 8)	50.0 % (4 of 8)
Methyl	80.0 % (4 of 5)	80.0 % (4 of 5)

1. MTx-HsTx1

VSCTGSKDCY APCRKQTGCP YGKCMNRKCK CNRC

Show sample condition

Sample

Temperature 300 (±0) K, pH 3 (±0)

#	Name	Isotope labeling	Type	Concentration
1	MTx-HsTx1			0.0 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WPD, Strand ID: A Detail

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Release date

2004-10-10

Citation

Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins
Regaya, I., Beeton, C., Ferrat, G., Andreotti, N., Darbon, H., De Waard, M., Sabatier, J.M.
J. Biol. Chem. (2004), 279, 55690-55696, PubMed 15498765 , DOI 10.1074/jbc.M410055200 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. MTx-HsTx1, : 1 : 138 entities Detail

Experiments performed 3 experiments Detail