BMRB: 6350

1H, 13C and 15N Chemical Shift Assignments for the LH1 Beta Polypeptide of Rhodospirillum rubrum

Authors

Wang, Z., Gokan, K., Kobayashi, M., Nozawa, T.

Assembly

LH1 Beta polypeptide

Entity

1. LH1 Beta polypeptide (polymer, Thiol state: not present), 55 monomers, 6148.968 Da Detail

AEVKQESLSG ITEGEAKEFH KIFTSSILVF FGVAAFAHLL VWIWRPWVPG PNGYS

Formula weight

6148.968 Da

Source organism

Rhodospirillum rubrum

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 73.1 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.1 % (484 of 662)	79.0 % (267 of 338)	62.2 % (166 of 267)	89.5 % (51 of 57)
Backbone	96.9 % (314 of 324)	96.4 % (108 of 112)	96.9 % (155 of 160)	98.1 % (51 of 52)
Sidechain	56.4 % (219 of 388)	70.4 % (159 of 226)	38.2 % (60 of 157)	0.0 % (0 of 5)
Aromatic	12.7 % (13 of 102)	25.5 % (13 of 51)	0.0 % (0 of 48)	0.0 % (0 of 3)
Methyl	63.6 % (42 of 66)	100.0 % (33 of 33)	27.3 % (9 of 33)

1. Beta subunit of LH1 complex

AEVKQESLSG ITEGEAKEFH KIFTSSILVF FGVAAFAHLL VWIWRPWVPG PNGYS

Show sample condition

Sample

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Beta subunit of LH1 complex	[U-95% 13C; U-95% 15N]		0.5 ~ 1.5 mM
2	chloroform-d:methanol-d3 [1:1 (v/v)]			100 %

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Calculated from 10 models in PDB: 1WRG, Strand ID: A Detail

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Release date

2005-03-16

Citation

Solution structures of the core light-harvesting alpha and beta polypeptides from Rhodospirillum rubrum: implications for the pigment-protein and protein-protein interactions
Wang, Z., Gokan, K., Kobayashi, M., Nozawa, T.
J. Mol. Biol. (2005), 347, 465-477, PubMed 15740753 , DOI 10.1016/j.jmb.2005.01.017 , Abstract

Related entities 1. LH1 Beta polypeptide, : 1 : 3 : 2 : 63 entities Detail

Experiments performed 8 experiments Detail