BMRB: 6354

1H, 13C and 15N resonance assignments and 15N-1H residual dipolar couplings for NECAP1 protein

Authors

Denisov, A.YU., Gehring, K.

Assembly

N-terminal domain of NECAP1 protein

Entity

1. N-terminal domain of NECAP1 protein (polymer, Thiol state: all free), 133 monomers, 15082.81 Da Detail

MAAELEYESV LCVKPDVSVY RIPPRASNRG YRASDWKLDQ PDWTGRLRIT SKGKIAYIKL EDKVSGELFA QAPVEQYPGI AVETVTDSSR YFVIRIQDGT GRSAFIGIGF TDRGDAFDFN VSLQDHFKWV KQE

Formula weight

15082.81 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 77.1 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.1 % (1206 of 1564)	70.2 % (571 of 813)	83.5 % (512 of 613)	89.1 % (123 of 138)
Backbone	95.4 % (750 of 786)	93.7 % (253 of 270)	96.9 % (377 of 389)	94.5 % (120 of 127)
Sidechain	63.4 % (571 of 901)	58.0 % (315 of 543)	72.9 % (253 of 347)	27.3 % (3 of 11)
Aromatic	26.6 % (42 of 158)	36.7 % (29 of 79)	13.2 % (10 of 76)	100.0 % (3 of 3)
Methyl	85.0 % (119 of 140)	85.7 % (60 of 70)	84.3 % (59 of 70)

1. NECAP1 protein

MAAELEYESV LCVKPDVSVY RIPPRASNRG YRASDWKLDQ PDWTGRLRIT SKGKIAYIKL EDKVSGELFA QAPVEQYPGI AVETVTDSSR YFVIRIQDGT GRSAFIGIGF TDRGDAFDFN VSLQDHFKWV KQE

Show sample condition

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	NECAP1 protein	[U-99% 13C; U-99% 15N]		1.4 mM
2	sodium phosphate buffer			25 mM
3	NaCl			75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	NECAP1 protein	[U-99% 15N]		1.4 mM
5	sodium phosphate buffer			25 mM
6	NaCl			75 mM

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LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 10 models in PDB: 1TQZ, Strand ID: A Detail

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RDC

121 RDC values in 1 lists
Field strength (¹H) 600 MHz, Temperature 303 (±0.5) K, pH 7.2 (±0.1) Detail

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Temperature 303 (±0.5) K, pH 7.2 (±0.1)

List #1 D_values_set_1, RDC code 1DNH, Field strength (¹H) 600 MHz

Release date

2006-01-07

Citation

The NECAP PHear domain increases clathrin accessory protein binding potential
Ritter, B., Denisov, A.YU., Philie, J., Allaire, P.D., Legendre-Guillemin, V., Zylbergold, P., Gehring, K., McPherson, P.S.
EMBO J. (2007), 26, 4066-4077, PubMed 17762867 , DOI 10.1038/sj.emboj.7601836 , Abstract

Related entities 1. N-terminal domain of NECAP1 protein, : 1 : 2 : 1 : 7 : 28 entities Detail

Interaction partners 1. N-terminal domain of NECAP1 protein, : 15 interactors Detail

Experiments performed 1 experiments Detail