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Found 1 Document (1.564 seconds)

BMRB: 6355

1XHJ

Solution Structure Of The Staphylococcus Epidermis Protein SE0936. Northest Strucutral Genomics Consortium Target SeR8.

Authors

Baran, M.C., Huang, Y.P., Acton, T., Xiao, R., Montelione, G.T.

Assembly

SE0936

Entity

1. SE0936 (polymer, Thiol state: all free), 88 monomers, 9801.026 Da Detail

MPTENPTMFD QVAEVIERLR PFLLRDGGDC TLVDVEDGIV KLQLHGACGT CPSSTITLKA GIERALHEEV PGVIEVEQVF LEHHHHHH

Formula weight

9801.026 Da

Source organism

Staphylococcus epidermidis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.0 %, Completeness: 89.5 %, Completeness (bb): 90.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.5 % (888 of 992)	90.4 % (461 of 510)	88.4 % (349 of 395)	89.7 % (78 of 87)
Backbone	90.5 % (469 of 518)	91.6 % (163 of 178)	89.9 % (231 of 257)	90.4 % (75 of 83)
Sidechain	88.8 % (493 of 555)	89.8 % (298 of 332)	87.7 % (192 of 219)	75.0 % (3 of 4)
Aromatic	56.5 % (35 of 62)	54.8 % (17 of 31)	58.1 % (18 of 31)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. SE0936

MPTENPTMFD QVAEVIERLR PFLLRDGGDC TLVDVEDGIV KLQLHGACGT CPSSTITLKA GIERALHEEV PGVIEVEQVF LEHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1XHJ, Strand ID: A Detail

Release date

2006-04-05

Citation

Solution Structure Of The Staphylococcus Epidermis Protein SE0936. Northest Strucutral Genomics Consortium Target SeR8
Baran, M.C., Huang, Y.P., Acton, T., Xiao, R., Montelione, G.T.

Related entities 1. SE0936, : 1 : 96 entities Detail

Experiments performed 6 experiments Detail

1 C13-HSQC

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	SE0936	[U-100% 13C; U-100% 15N]	8 mM
10	NaN3		0.02 %
11	DTT		10 mM
12	CaCl2		5 mM
13	NaCl		100 mM
14	MES		20 mM
15	D2O		5 %
16	H20		95 %

2 NH-HSQC

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	SE0936	[U-100% 13C; U-100% 15N]	8 mM
10	NaN3		0.02 %
11	DTT		10 mM
12	CaCl2		5 mM
13	NaCl		100 mM
14	MES		20 mM
15	D2O		5 %
16	H20		95 %

3 HNCO

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	SE0936	[U-100% 13C; U-100% 15N]	8 mM
10	NaN3		0.02 %
11	DTT		10 mM
12	CaCl2		5 mM
13	NaCl		100 mM
14	MES		20 mM
15	D2O		5 %
16	H20		95 %

4 HNCAB

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	SE0936	[U-100% 13C; U-100% 15N]	8 mM
10	NaN3		0.02 %
11	DTT		10 mM
12	CaCl2		5 mM
13	NaCl		100 mM
14	MES		20 mM
15	D2O		5 %
16	H20		95 %

5 N15-NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	SE0936	[U-100% 13C; U-100% 15N]	8 mM
10	NaN3		0.02 %
11	DTT		10 mM
12	CaCl2		5 mM
13	NaCl		100 mM
14	MES		20 mM
15	D2O		5 %
16	H20		95 %

6 C13-NOESY

Instrument

Model Bruker AVANCE (800 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SE0936	[U-5% 13C; U-100% 15N]	8 mM
2	NaN3		0.02 %
3	DTT		10 mM
4	CaCl2		5 mM
5	NaCl		100 mM
6	MES		20 mM
7	D2O		5 %
8	H20		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	SE0936	[U-100% 13C; U-100% 15N]	8 mM
10	NaN3		0.02 %
11	DTT		10 mM
12	CaCl2		5 mM
13	NaCl		100 mM
14	MES		20 mM
15	D2O		5 %
16	H20		95 %

nullKeywords Alpha-beta, NifU-like, Structural Genomics, Protein Structure Initiative, NESG

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Found 1 Document (1.564 seconds)

BMRB: 6355

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SE0936, : 1 : 96 entities Detail

Experiments performed 6 experiments Detail

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