BMRB: 6356

Yeast frataxin solution structure

Authors

He, Y., Alam, S.A., Proteasa, S., Zhang, Y., Lesuisse, E., Dancis, A., Stemmler, T.

Assembly

Yeast Frataxin homolog 1 protein

Entity

1. Yeast Frataxin homolog 1 protein (polymer, Thiol state: all free), 123 monomers, 13783.24 Da Detail

MESSTDGQVV PQEVLNLPLE KYHEEADDYL DHLLDSLEEL SEAHPDCIPD VELSHGVMTL EIPAFGTYVI NKQPPNKQIW LASPLSGPNR FDLLNGEWVS LRNGTKLTDI LTEEVEKAIS KSQ

Formula weight

13783.24 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 83.3 %, Completeness (bb): 95.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.3 % (1186 of 1423)	78.5 % (580 of 739)	88.9 % (495 of 557)	87.4 % (111 of 127)
Backbone	95.1 % (685 of 720)	96.3 % (234 of 243)	94.2 % (342 of 363)	95.6 % (109 of 114)
Sidechain	74.8 % (613 of 820)	69.8 % (346 of 496)	85.2 % (265 of 311)	15.4 % (2 of 13)
Aromatic	46.4 % (39 of 84)	66.7 % (28 of 42)	22.5 % (9 of 40)	100.0 % (2 of 2)
Methyl	95.2 % (139 of 146)	94.5 % (69 of 73)	95.9 % (70 of 73)

1. Saccharomyces cerevisiae

MESSTDGQVV PQEVLNLPLE KYHEEADDYL DHLLDSLEEL SEAHPDCIPD VELSHGVMTL EIPAFGTYVI NKQPPNKQIW LASPLSGPNR FDLLNGEWVS LRNGTKLTDI LTEEVEKAIS KSQ

Show sample condition

Sample

Temperature 298 (±0.05) K, pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Saccharomyces cerevisiae			1 mM
2	sodium phosphate buffer			5 mM
3	DTT			2 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 2GA5, Strand ID: A Detail

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Release date

2005-05-02

Citation

Yeast frataxin solution structure, iron binding, and ferrochelatase interaction
He, Y., Alam, S.A., Proteasa, S.V., Zhang, Y., Lesuisse, E., Dancis, A., Stemmler, T.L.
Biochemistry (2004), 43, 16254-16262, PubMed 15610019 , DOI 10.1021/bi0488193 , Abstract

Related entities 1. Yeast Frataxin homolog 1 protein, : 1 : 2 : 51 entities Detail

Interaction partners 1. Yeast Frataxin homolog 1 protein, : 10 interactors Detail

Experiments performed 10 experiments Detail