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BMRB: 6358

1XS3

1H, 13C, and 15N Chemical Shift Assignments for a bacterial BolA-like protein XC975

Authors

Chin, K., Lin, F., Hu, Y., Sze, K., Lyu, P., Chou, S.

Assembly

Xcc975 monomer

Entity

1. Xcc975 monomer (polymer, Thiol state: not present), 80 monomers, 8677.941 Da Detail

MRKRPLDAET IRKLIESGLP EARVDVQGED GVHFEATVVS PAFVGKAPLA RHRMVYATLG ELMGGAIHAL QLKTLTPDEA

Formula weight

8677.941 Da

Source organism

Xanthomonas campestris

Exptl. method

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 83.9 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (755 of 900)	83.9 % (391 of 466)	82.1 % (293 of 357)	92.2 % (71 of 77)
Backbone	91.1 % (428 of 470)	91.4 % (148 of 162)	89.7 % (209 of 233)	94.7 % (71 of 75)
Sidechain	78.5 % (395 of 503)	79.9 % (243 of 304)	77.2 % (152 of 197)	0.0 % (0 of 2)
Aromatic	35.0 % (14 of 40)	60.0 % (12 of 20)	10.0 % (2 of 20)
Methyl	93.4 % (99 of 106)	96.2 % (51 of 53)	90.6 % (48 of 53)

1. XC975 protein

MRKRPLDAET IRKLIESGLP EARVDVQGED GVHFEATVVS PAFVGKAPLA RHRMVYATLG ELMGGAIHAL QLKTLTPDEA

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

LACS Plot; CA

Referencing offset: 1.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 1.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1XS3, Strand ID: A Detail

Release date

2005-03-17

Citation

NMR structure note--solution structure of a bacterial BolA-like protein XC975 from a plant pathogen Xanthomonas campestris pv. campestris
Chin, K., Lin, F., Hu, Y., Sze, K., Lyu, P., Chou, S.
J. Biomol. NMR (2005), 31, 167-172, PubMed 15772757 , DOI 10.1007/s10858-004-7804-9 , Abstract

Related entities 1. Xcc975 monomer, : 1 : 49 entities Detail

Experiments performed 23 experiments Detail

1 1H15N HSQC

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

2 1H 13C HSQC

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

3 HNCACB

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

4 CBCA(CO)NH

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

5 H(CCO)NH-TOCSY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

6 C(CO)NH-TOCSY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

7 NOESYNHSQC

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

8 NOESYCHSQC

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

9 HCCH-TOCSY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

10 HCCH-COSY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

11 HBCBCGCDCEHE

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

12 HBCBCGCDHD

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

13 HNHB

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

14 HNHA

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

15 HBHA(CO)NH

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

16 HNCO

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

17 ME-TOCSY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

18 DQ-COSY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

19 2D-NOESY

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

20 10%13C-CTHSQC

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

21 NOESYHSQCCH3NH2

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

22 CTHSQC

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

23 HNCACO

Instrument

Varian 2.2K - 800 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	XC975 protein	[U-13C; U-15N]		2.5 mM

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