BMRB: 6366

1H, 13C, and 15N Chemical Shift Assignments of Bacillus subtilis Protein yqbG: The Northeast Structural Genomics Consortium Target SR215

Authors

Shen, Y., Liu, G., Ma, L., Acton, T., Atreya, H.S., Xiao, R., Joachimiak, A., Montelione, G.T., Szyperski, T.

Assembly

Hypothetical protein yqbG

Entity

1. Hypothetical protein yqbG (polymer, Thiol state: not present), 139 monomers, 15751.74 Da Detail

MLLITPDELK SYSVFESVKT RPDELLKQDI LEATADIILK VGHDFSDAEY IPLPETVRLA LLKLSQFYAL INGDESIIKG YTTEKIGDYS YTLGDGSSLQ KPDVYALIKD YVKPADPDLE GIEAKVRMRS ILEHHHHHH

Formula weight

15751.74 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 85.3 %, Completeness (bb): 78.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.3 % (1398 of 1639)	91.9 % (779 of 848)	75.6 % (495 of 655)	91.2 % (124 of 136)
Backbone	78.0 % (640 of 820)	91.7 % (255 of 278)	64.6 % (265 of 410)	90.9 % (120 of 132)
Sidechain	92.5 % (880 of 951)	91.9 % (524 of 570)	93.4 % (352 of 377)	100.0 % (4 of 4)
Aromatic	77.9 % (95 of 122)	73.8 % (45 of 61)	82.0 % (50 of 61)
Methyl	97.8 % (174 of 178)	97.8 % (87 of 89)	97.8 % (87 of 89)

1. Hypothetical protein yqbG

MLLITPDELK SYSVFESVKT RPDELLKQDI LEATADIILK VGHDFSDAEY IPLPETVRLA LLKLSQFYAL INGDESIIKG YTTEKIGDYS YTLGDGSSLQ KPDVYALIKD YVKPADPDLE GIEAKVRMRS ILEHHHHHH

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical protein yqbG	[U-13C; U-15N]		0.94 mM

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LACS Plot; CA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1ZTS, Strand ID: A Detail

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Release date

2005-12-27

Citation

NMR structure of protein yqbG from Bacillus subtilis reveals a novel alpha-helical protein fold
Liu, G., Shen, Y., Xiao, R., Acton, T., Ma, L., Joachimiak, A., Montelione, G.T., Szyperski, T.
Proteins (2006), 62, 288-291, PubMed 16281282 , DOI 10.1002/prot.20666 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Hypothetical protein yqbG, : 1 : 2 : 2 entities Detail

Experiments performed 5 experiments Detail