BMRB: 6377

Backbone 1H, 13C, and 15N Chemical Shift Assignments for feline prion protein

Authors

Lysek, D.A., Nivon, L.G., Wuthrich, K.

Assembly

fPrP monomer

Entity

1. fPrP monomer (polymer, Thiol state: all disulfide bound), 113 monomers, 13321.66 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDQ YSNQNNFVHD CVNITVRQHT VTTTTKGENF TETDMKIMER VVEQMCVTQY QKESEAYYQR RAS

Formula weight

13321.66 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Felis catus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.9 %, Completeness (bb): 82.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.9 % (1141 of 1329)	94.5 % (655 of 693)	72.0 % (367 of 510)	94.4 % (119 of 126)
Backbone	82.4 % (554 of 672)	96.5 % (223 of 231)	68.3 % (226 of 331)	95.5 % (105 of 110)
Sidechain	89.8 % (684 of 762)	93.5 % (432 of 462)	83.8 % (238 of 284)	87.5 % (14 of 16)
Aromatic	75.4 % (104 of 138)	97.1 % (67 of 69)	53.6 % (37 of 69)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. fPrP

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDQ YSNQNNFVHD CVNITVRQHT VTTTTKGENF TETDMKIMER VVEQMCVTQY QKESEAYYQR RAS

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 4.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fPrP	[U-13C; U-15N]		1 mM
2	sodium acidate			10 mM

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LACS Plot; CA

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XYJ, Strand ID: A Detail

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Release date

2004-11-04

Citation

Prion protein NMR structures of cats, dogs, pigs, and sheep
Lysek, D.A., Schorn, C., Nivon, L.G., Esteve-Moya, V., Christen, B., Calzolai, L., von Schroetter, C., Fiorito, F., Herrmann, T., Guntert, P., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 640-645, PubMed 15647367 , DOI 10.1073/pnas.0408937102 , Abstract

Related entities 1. fPrP monomer, : 1 : 1 : 158 entities Detail

Interaction partners 1. fPrP monomer, : 1 interactors Detail

Experiments performed 3 experiments Detail