BMRB: 6378

canine prion protein

Authors

Lysek, D.A., Esteve-Moya, V., Schorn, C.

Assembly

canine prion protein

Entity

1. canine prion protein (polymer, Thiol state: all disulfide bound), 113 monomers, 13214.54 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP DQVYYRPVDQ YSNQNNFVRD CVNITVKQHT VTTTTKGENF TETDMKIMER VVEQMCVTQY QKESEAYYQR GAS

Formula weight

13214.54 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Canis lupus familiaris

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.5 %, Completeness (bb): 81.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.5 % (1143 of 1322)	95.9 % (661 of 689)	72.8 % (370 of 508)	89.6 % (112 of 125)
Backbone	81.3 % (546 of 672)	95.3 % (221 of 232)	67.9 % (224 of 330)	91.8 % (101 of 110)
Sidechain	92.4 % (697 of 754)	96.3 % (440 of 457)	87.2 % (246 of 282)	73.3 % (11 of 15)
Aromatic	76.1 % (102 of 134)	98.5 % (66 of 67)	53.7 % (36 of 67)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. canine prion protein

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP DQVYYRPVDQ YSNQNNFVRD CVNITVKQHT VTTTTKGENF TETDMKIMER VVEQMCVTQY QKESEAYYQR GAS

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 4.5 (±0.05), Details canine prion protein sodium acetate pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	canine prion protein	[U-13C; U-15N]		1 mM
2	sodium acetate			0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XYK, Strand ID: A Detail

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Release date

2004-11-04

Citation

Prion protein NMR structures of cats, dogs, pigs, and sheep
Lysek, D.A., Schorn, C., Nivon, L.G., Esteve-Moya, V., Christen, B., Calzolai, L., von Schroetter, C., Fiorito, F., Herrmann, T., Guntert, P., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 640-645, PubMed 15647367 , DOI 10.1073/pnas.0408937102 , Abstract

Related entities 1. canine prion protein, : 1 : 1 : 162 entities Detail

Interaction partners 1. canine prion protein, : 1 interactors Detail

Experiments performed 3 experiments Detail