BMRB: 6380

1H, 13C, and 15N Chemical Shift Assignments for pig prion protein

Authors

Schorn, C., Lysek, D.A., Wuthrich, K.

Assembly

pig prion protein monomer

Entity

1. pig prion protein monomer (polymer, Thiol state: all disulfide bound), 113 monomers, 13106.42 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGSDYEDR YYRENMYRYP NQVYYRPVDQ YSNQNSFVHD CVNITVKQHT VTTTTKGENF TETDVKMIER VVEQMCITQY QKEYEAYAQR GAS

Formula weight

13106.42 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Sus scrofa

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.3 %, Completeness (bb): 80.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.3 % (1070 of 1316)	90.9 % (621 of 683)	64.6 % (329 of 509)	96.8 % (120 of 124)
Backbone	80.4 % (540 of 672)	98.3 % (228 of 232)	62.1 % (205 of 330)	97.3 % (107 of 110)
Sidechain	82.8 % (619 of 748)	87.1 % (393 of 451)	75.3 % (213 of 283)	92.9 % (13 of 14)
Aromatic	48.6 % (67 of 138)	50.7 % (35 of 69)	46.4 % (32 of 69)
Methyl	85.7 % (84 of 98)	91.8 % (45 of 49)	79.6 % (39 of 49)

1. pig prion protein

GSVVGGLGGY MLGSAMSRPL IHFGSDYEDR YYRENMYRYP NQVYYRPVDQ YSNQNSFVHD CVNITVKQHT VTTTTKGENF TETDVKMIER VVEQMCITQY QKEYEAYAQR GAS

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 4.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	pig prion protein	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: 0.43 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.43 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XYQ, Strand ID: A Detail

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Release date

2004-11-08

Citation

Prion protein NMR structures of cats, dogs, pigs, and sheep
Lysek, D.A., Schorn, C., Nivon, L.G., Esteve-Moya, V., Christen, B., Calzolai, L., von Schroetter, C., Fiorito, F., Herrmann, T., Guntert, P., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 640-645, PubMed 15647367 , DOI 10.1073/pnas.0408937102 , Abstract

Related entities 1. pig prion protein monomer, : 1 : 1 : 162 entities Detail

Interaction partners 1. pig prion protein monomer, : 1 interactors Detail

Experiments performed 3 experiments Detail