BMRB: 6382

1H, 13C, and 15N Chemical Shift Assignments for Xenopus prion protein.

Authors

Perez, D.R., Wuthrich, K.

Assembly

prion protein

Entity

1. prion protein (polymer, Thiol state: all disulfide bound), 130 monomers, 15004.75 Da Detail

GSGYNKQWKP PKSKTNMKSV AIGAAAGAIG GYMLGNAVGR MSYQFNNPME SRYYNDYYNQ MPNRVYRPMY RGEEYVSEDR FVRDCYNMSV TEYIIKPAEG KNNSELNQLD TTVKSQIIRE MCITEYRRGS

Formula weight

15004.75 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS85:SG	1:CYS122:SG

Source organism

Xenopus laevis

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.0 %, Completeness (bb): 85.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.0 % (1379 of 1532)	98.5 % (797 of 809)	76.1 % (442 of 581)	98.6 % (140 of 142)
Backbone	85.7 % (658 of 768)	98.9 % (262 of 265)	72.3 % (274 of 379)	98.4 % (122 of 124)
Sidechain	95.0 % (839 of 883)	98.3 % (535 of 544)	89.1 % (286 of 321)	100.0 % (18 of 18)
Aromatic	77.2 % (105 of 136)	100.0 % (68 of 68)	53.7 % (36 of 67)	100.0 % (1 of 1)
Methyl	97.8 % (90 of 92)	95.7 % (44 of 46)	100.0 % (46 of 46)

1. prion

GSGYNKQWKP PKSKTNMKSV AIGAAAGAIG GYMLGNAVGR MSYQFNNPME SRYYNDYYNQ MPNRVYRPMY RGEEYVSEDR FVRDCYNMSV TEYIIKPAEG KNNSELNQLD TTVKSQIIRE MCITEYRRGS

Show sample condition

Sample

Temperature 293.15 (±0.2) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	prion	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XU0, Strand ID: A Detail

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Release date

2004-11-09

Citation

NMR assignment of the Xenopus leavis prion protein fragment xlPrP(98-226)
Perez, D.R., Wuthrich, K.
J. Biomol. NMR (2005), 31, 260-260

Related entities 1. prion protein, : 1 : 138 entities Detail

Experiments performed 5 experiments Detail