BMRB: 6383

NMR solution structure of the recombinant elk and mouse/elk variant prion proteins

Authors

Gossert, A.D., Bonjour, S., Lysek, D.A.

Assembly

eprp monomer

Entity

1. eprp monomer (polymer, Thiol state: all disulfide bound), 113 monomers, 13250.58 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDQ YNNQNTFVHD CVNITVKQHT VTTTTKGENF TETDIKMMER VVEQMCITQY QRESEAYYQR GAS

Formula weight

13250.58 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Cervus canadensis nelsoni

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 85.3 %, Completeness (bb): 83.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.3 % (1130 of 1324)	97.8 % (674 of 689)	65.4 % (333 of 509)	97.6 % (123 of 126)
Backbone	83.3 % (560 of 672)	97.8 % (227 of 232)	68.5 % (226 of 330)	97.3 % (107 of 110)
Sidechain	88.9 % (672 of 756)	97.8 % (447 of 457)	73.9 % (209 of 283)	100.0 % (16 of 16)
Aromatic	49.3 % (68 of 138)	98.6 % (68 of 69)	0.0 % (0 of 69)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. elk prion protein

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDQ YNNQNTFVHD CVNITVKQHT VTTTTKGENF TETDIKMMER VVEQMCITQY QRESEAYYQR GAS

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 4.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	elk prion protein	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: 0.45 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.45 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1XYW, Strand ID: A Detail

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Release date

2004-11-09

Citation

Prion protein NMR structures of the elk and of mouse/elk hybrids
Gossert, A.D., Bonjour, S., Lysek, D.A., Fiorito, F., Wuthrich, K.
Proc. Natl. Acad. Sci. U.S.A. (2005), 102, 646-650, PubMed , DOI:

Related entities 1. eprp monomer, : 1 : 2 : 1 : 159 entities Detail

Interaction partners 1. eprp monomer, : 1 interactors Detail

Experiments performed 3 experiments Detail