BMRB: 6387

Structural and Functional Study of Anemonia Elastase Inhibitor, a "Non-classical" Kazal-type Inhibitor from Anemonia sulcata

Authors

Hemmi, H., Kumazaki, T., Yoshizawa-Kumagaye, K., Nishiuchi, Y., Yoshida, T., Ohkubo, T., Kobayashi, Y.

Assembly

aei-analogue monomer

Entity

1. aei-analogue monomer (polymer, Thiol state: all disulfide bound), 48 monomers, 5105.889 Da Detail

KPDAPCICTM QYDPVCGSDG ITYGNACMLL CASARSDTPI ELVHKGRC

Formula weight

5105.889 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS31:SG
2	disulfide	sing	1:CYS8:SG	1:CYS27:SG
3	disulfide	sing	1:CYS16:SG	1:CYS48:SG

Source organism

Anemonia sulcata

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.6 %, Completeness (bb): 99.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.6 % (261 of 262)	99.6 % (261 of 262)
Backbone	99.0 % (95 of 96)	99.0 % (95 of 96)
Sidechain	100.0 % (166 of 166)	100.0 % (166 of 166)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (23 of 23)	100.0 % (23 of 23)

1. anemonia elastase inhibitor analogue

KPDAPCICTM QYDPVCGSDG ITYGNACMLL CASARSDTPI ELVHKGRC

Show sample condition

Sample

Temperature 298 (±1) K, pH 2.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	anemonia elastase inhibitor analogue			3.0 ~ 3.5 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Y1C, Strand ID: A Detail

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Release date

2004-11-14

Citation

Structural and functional study of an Anemonia elastase inhibitor, a "nonclassical" Kazal-type inhibitor from Anemonia sulcata
Hemmi, H., Kumazaki, T., Yoshizawa-Kumagaye, K., Nishiuchi, Y., Yoshida, T., Ohkubo, T., Kobayashi, Y.
Biochemistry (2005), 44, 9626-9636, PubMed 16008348 , DOI 10.1021/bi0472806 , Abstract

Related entities 1. aei-analogue monomer, : 1 : 2 : 312 entities Detail

Experiments performed 6 experiments Detail