BMRBj - BMREntryMaster

Found 1 Document (1.576 seconds)

BMRB: 6390

2AGM

NMR assignment of the R-module from the Azotobacter vinelandii mannuronan C5-epimerase AlgE4

Authors

Aachmann, F.L., Svanem, B.GLARUM., Valla, S., Petersen, S.BJORN., Wimmer, R.

Assembly

R-module

Entity

1. R-module (polymer, Thiol state: not present), 167 monomers, 16963.90 Da Detail

GSDGEPLVGG DTDDQLQGGS GADRLDGGAG DDILDGGAGR DRLSGGAGAD TFVFSAREDS YRTDTAVFND LILDFEASED RIDLSALGFS GLGDGYGGTL LLKTNAEGTR TYLKSFEADA EGRRFEVALD GDHTGDLSAA NVVFAATGTT TELEVLGDSG TQAGAIV

2. CA (non-polymer), 40.078 Da

Total weight

17003.979 Da

Max. entity weight

16963.9 Da

Source organism

Azotobacter vinelandii

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, torsion angle dynamics, energy-minimisation

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.4 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.4 % (1466 of 1677)	87.7 % (741 of 845)	85.5 % (564 of 660)	93.6 % (161 of 172)
Backbone	96.0 % (960 of 1000)	96.1 % (349 of 363)	96.8 % (456 of 471)	93.4 % (155 of 166)
Sidechain	78.6 % (640 of 814)	81.3 % (392 of 482)	74.2 % (242 of 326)	100.0 % (6 of 6)
Aromatic	22.2 % (24 of 108)	44.4 % (24 of 54)	0.0 % (0 of 54)
Methyl	93.5 % (174 of 186)	94.6 % (88 of 93)	92.5 % (86 of 93)

1. R-module

GSDGEPLVGG DTDDQLQGGS GADRLDGGAG DDILDGGAGR DRLSGGAGAD TFVFSAREDS YRTDTAVFND LILDFEASED RIDLSALGFS GLGDGYGGTL LLKTNAEGTR TYLKSFEADA EGRRFEVALD GDHTGDLSAA NVVFAATGTT TELEVLGDSG TQAGAIV

Show sample condition

Sample #1

Temperature 298 (±0.2) K, pH 6.9 (±0.1), Details 95 % H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	R-module	[U-99% 13C; U-99% 15N]	1.6 mM
2	CALCIUM (II) ION		1.6 mM
3	CaCl2		50 mM
4	Na-HEPES		20 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	R-module	[U-99% 13C; U-99% 15N]	0.8 ~ 1.1 mM
6	CALCIUM (II) ION		0.8 ~ 1.1 mM
7	CaCl2		50 mM
8	Na-HEPES		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.06 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2AGM, Strand ID: A Detail

Release date

2005-04-07

Citation

NMR assignment of the R-module from the Azotobacter vinelandii Mannuronan C5-epimerase AlgE4
Aachmann, F.L., Svanem, B.GLAERUM., Valla, S., Petersen, S.BJORN., Wimmer, R.
J. Biomol. NMR (2005), 31, 259-259, PubMed 15803398 , DOI 10.1007/s10858-004-7911-7 , Abstract

Related entities 1. R-module, : 1 : 1 : 26 entities Detail

Experiments performed 5 experiments Detail

1 Aromatic side-chain atoms were assigned from a series of 2D H(CC)H-COSY, TOCSY

Instrument

Model Bruker DRX (600 MHz) Details Bruker DRX600 spectrometer equipped with a 5-mm xyz-grad TXI(H/C/N) probe.

Sample #1

Temperature 298 (±0.2) K, pH 6.9 (±0.1), Details 95 % H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	R-module	[U-99% 13C; U-99% 15N]	1.6 mM
2	CALCIUM (II) ION		1.6 mM
3	CaCl2		50 mM
4	Na-HEPES		20 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	R-module	[U-99% 13C; U-99% 15N]	0.8 ~ 1.1 mM
6	CALCIUM (II) ION		0.8 ~ 1.1 mM
7	CaCl2		50 mM
8	Na-HEPES		20 mM

2 and NOESY experiments optimized for aromatic signals.

Instrument

Model Bruker DRX (600 MHz) Details Bruker DRX600 spectrometer equipped with a 5-mm xyz-grad TXI(H/C/N) probe.

Sample #1

Temperature 298 (±0.2) K, pH 6.9 (±0.1), Details 95 % H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	R-module	[U-99% 13C; U-99% 15N]	1.6 mM
2	CALCIUM (II) ION		1.6 mM
3	CaCl2		50 mM
4	Na-HEPES		20 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	R-module	[U-99% 13C; U-99% 15N]	0.8 ~ 1.1 mM
6	CALCIUM (II) ION		0.8 ~ 1.1 mM
7	CaCl2		50 mM
8	Na-HEPES		20 mM

3 Due to the high content of Glycine and Alanine residues, MUSIC spectra have

Instrument

Model Bruker DRX (600 MHz) Details Bruker DRX600 spectrometer equipped with a 5-mm xyz-grad TXI(H/C/N) probe.

Sample #1

Temperature 298 (±0.2) K, pH 6.9 (±0.1), Details 95 % H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	R-module	[U-99% 13C; U-99% 15N]	1.6 mM
2	CALCIUM (II) ION		1.6 mM
3	CaCl2		50 mM
4	Na-HEPES		20 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	R-module	[U-99% 13C; U-99% 15N]	0.8 ~ 1.1 mM
6	CALCIUM (II) ION		0.8 ~ 1.1 mM
7	CaCl2		50 mM
8	Na-HEPES		20 mM

4 been very helpful to identify Gly/Ala residues and residues following after

Instrument

Model Bruker DRX (600 MHz) Details Bruker DRX600 spectrometer equipped with a 5-mm xyz-grad TXI(H/C/N) probe.

Sample #1

Temperature 298 (±0.2) K, pH 6.9 (±0.1), Details 95 % H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	R-module	[U-99% 13C; U-99% 15N]	1.6 mM
2	CALCIUM (II) ION		1.6 mM
3	CaCl2		50 mM
4	Na-HEPES		20 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	R-module	[U-99% 13C; U-99% 15N]	0.8 ~ 1.1 mM
6	CALCIUM (II) ION		0.8 ~ 1.1 mM
7	CaCl2		50 mM
8	Na-HEPES		20 mM

5 Gly/Ala.

Instrument

Model Bruker DRX (600 MHz) Details Bruker DRX600 spectrometer equipped with a 5-mm xyz-grad TXI(H/C/N) probe.

Sample #1

Temperature 298 (±0.2) K, pH 6.9 (±0.1), Details 95 % H2O, 5% D2O

#	Name	Isotope labeling	Concentration
1	R-module	[U-99% 13C; U-99% 15N]	1.6 mM
2	CALCIUM (II) ION		1.6 mM
3	CaCl2		50 mM
4	Na-HEPES		20 mM

Sample #2

Temperature 298 (±0.2) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	R-module	[U-99% 13C; U-99% 15N]	0.8 ~ 1.1 mM
6	CALCIUM (II) ION		0.8 ~ 1.1 mM
7	CaCl2		50 mM
8	Na-HEPES		20 mM

nullKeywords Alginate, Azotobacter vinelandii, R-module, Mannuronan C5-epimerase

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.576 seconds)

BMRB: 6390

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. R-module, : 1 : 1 : 26 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2