BMRBj - BMREntryMaster

Found 1 Document (1.802 seconds)

BMRB: 6392

1O7B

Refined solution structure of the human TSG-6 Link module

Authors

Blundell, C.D., Teriete, P., Kahmann, J.D., Pickford, A.R., Campbell, I.D.

Assembly

human TSG-6

Entity

1. human TSG-6 (polymer, Thiol state: all disulfide bound), 98 monomers, 10926.41 Da Detail

GVYHREARSG KYKLTYAEAK AVCEFEGGHL ATYKQLEAAR KIGFHVCAAG WMAKGRVGYP IVKPGPNCGF GKTGIIDYGI RLNRSERWDA YCYNPHAK

Formula weight

10926.41 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS23:SG	1:CYS92:SG
2	disulfide	sing	1:CYS47:SG	1:CYS68:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

AB INITIO SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.3 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.3 % (1083 of 1136)	97.8 % (579 of 592)	91.7 % (407 of 444)	97.0 % (97 of 100)
Backbone	96.9 % (562 of 580)	98.5 % (202 of 205)	95.7 % (269 of 281)	96.8 % (91 of 94)
Sidechain	94.2 % (604 of 641)	97.4 % (377 of 387)	89.1 % (221 of 248)	100.0 % (6 of 6)
Aromatic	82.8 % (111 of 134)	95.5 % (64 of 67)	69.2 % (45 of 65)	100.0 % (2 of 2)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. human TSG-6

GVYHREARSG KYKLTYAEAK AVCEFEGGHL ATYKQLEAAR KIGFHVCAAG WMAKGRVGYP IVKPGPNCGF GKTGIIDYGI RLNRSERWDA YCYNPHAK

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Concentration
1	human TSG-6	1.0 mM
2	Na ions	2 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	human TSG-6	[U-15N]		2.0 mM
5	Na ions			2 mM

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	human TSG-6	[U-15N; U-13C]		2.3 mM
7	Na ions			2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1O7B, Strand ID: T Detail

Release date

2005-11-13

Citation

Towards a structure for a TSG-6.hyaluronan complex by modeling and NMR spectroscopy: insights into other members of the link module superfamily
Blundell, C.D., Almond, A., Mahoney, D.J., Deangelis, P.L., Campbell, I.D., Day, A.J.
J. Biol. Chem. (2005), 280, 18189-18201, PubMed 15718240 , DOI 10.1074/jbc.M414343200 , Abstract

Related entities 1. human TSG-6, : 1 : 5 : 1 : 249 entities Detail

Interaction partners 1. human TSG-6, : 26 interactors Detail

More details for 26 interactors identified by 5 citations

Experiments performed 4 experiments Detail

1 15N-NOESY-HSQC

Instrument

#1 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 600MHz.

#2 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 750MHz.

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Concentration
1	human TSG-6	1.0 mM
2	Na ions	2 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	human TSG-6	[U-15N]		2.0 mM
5	Na ions			2 mM

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	human TSG-6	[U-15N; U-13C]		2.3 mM
7	Na ions			2 mM

2 15N-HSQC-NOESY-HSQC

Instrument

#1 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 600MHz.

#2 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 750MHz.

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Concentration
1	human TSG-6	1.0 mM
2	Na ions	2 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	human TSG-6	[U-15N]		2.0 mM
5	Na ions			2 mM

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	human TSG-6	[U-15N; U-13C]		2.3 mM
7	Na ions			2 mM

3 13C-NOESY-HSQC

Instrument

#1 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 600MHz.

#2 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 750MHz.

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Concentration
1	human TSG-6	1.0 mM
2	Na ions	2 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	human TSG-6	[U-15N]		2.0 mM
5	Na ions			2 mM

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	human TSG-6	[U-15N; U-13C]		2.3 mM
7	Na ions			2 mM

4 2D-NOESY

Instrument

#1 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 600MHz.

#2 Model na n/a (750 MHz) Details Homebuilt Spectrometer incorporating Oxford Instruments Magnet, 750MHz.

Sample #1

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Concentration
1	human TSG-6	1.0 mM
2	Na ions	2 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	human TSG-6	[U-15N]		2.0 mM
5	Na ions			2 mM

Sample #3

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
6	human TSG-6	[U-15N; U-13C]		2.3 mM
7	Na ions			2 mM

nullKeywords hyaluronan-binding domain, carbohydrate-binding domain, Link module

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Found 1 Document (1.802 seconds)

BMRB: 6392

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Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. human TSG-6, : 1 : 5 : 1 : 249 entities Detail

Interaction partners 1. human TSG-6, : 26 interactors Detail

Experiments performed 4 experiments Detail

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