BMRB: 6396

Chemical shift Assignment for mouse Roadblock/LC7 domain BC029172

Authors

Song, J., Vinarov, D.A., Tyler, E.M., Shahan, M.N., Loushin Newman, C., Tyler, R.C., Lee, M.S., Markley, J.L.

Assembly

BC029172

Entity

1. BC029172 (polymer, Thiol state: not present), 104 monomers, 11980.50 Da Detail

GHHHHHHLEA EVEETLKRLQ SQKGVQGIIV VNTEGIPIKS TMDNPTTTQY ANLMHNFILK ARSTVREIDP QNDLTFLRIR SKKNEIMVAP DKDYFLIVIQ NPTE

Formula weight

11980.5 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 82.7 %, Completeness (bb): 93.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.7 % (1030 of 1246)	81.0 % (527 of 651)	82.8 % (400 of 483)	92.0 % (103 of 112)
Backbone	93.6 % (575 of 614)	94.2 % (195 of 207)	93.2 % (287 of 308)	93.9 % (93 of 99)
Sidechain	75.3 % (551 of 732)	74.8 % (332 of 444)	76.0 % (209 of 275)	76.9 % (10 of 13)
Aromatic	13.5 % (10 of 74)	27.0 % (10 of 37)	0.0 % (0 of 37)
Methyl	95.2 % (120 of 126)	96.8 % (61 of 63)	93.7 % (59 of 63)

1. BC029172

GHHHHHHLEA EVEETLKRLQ SQKGVQGIIV VNTEGIPIKS TMDNPTTTQY ANLMHNFILK ARSTVREIDP QNDLTFLRIR SKKNEIMVAP DKDYFLIVIQ NPTE

Show sample condition

Sample

Pressure 1 (±0.05) atm, Temperature 298 (±0.2) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	BC029172	[U-15N; U-13C]		1 mM
2	Sodium Chloride			100 mM
3	Sodium Acetate			10 mM

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.18 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Y4O, Strand ID: A, B Detail

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Release date

2007-01-28

Citation

Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex
Song, J., Tyler, R.C., Lee, M.S., Tyler, E.M., Markley, J.L.
J. Mol. Biol. (2005), 354, 1043-1051, PubMed 16289575 , DOI 10.1016/j.jmb.2005.10.017 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. BC029172, : 1 : 3 : 78 entities Detail

Interaction partners 1. BC029172, : 2 interactors Detail

Experiments performed 2 experiments Detail