BMRB: 6398

Sulfolobus Solfataricus Acylphosphatase 1H chemical shift assignment

Authors

Corazza, A., Pagano, K., Viglino, P., Esposito, G.

Assembly

Sulfolobus solfataricus acylphosphatase

Entity

1. Sulfolobus solfataricus acylphosphatase (polymer, Thiol state: not present), 103 monomers, 11780.30 Da Detail

GSMKKWSDTE VFEMLKRMYA RVYGLVQGVG FRKFVQIHAI RLGIKGYAKN LPDGSVEVVA EGYEEALSKL LERIKQGPPA AEVEKVDYSF SEYKGEFEDF ETY

Formula weight

11780.3 Da

Source organism

Saccharolobus solfataricus

Exptl. method

NMR

Refine. method

restrained minimization

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 75.3 %, Completeness (bb): 95.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	75.3 % (566 of 752)	72.0 % (466 of 647)	95.2 % (100 of 105)
Backbone	95.5 % (299 of 313)	95.3 % (203 of 213)	96.0 % (96 of 100)
Sidechain	60.8 % (267 of 439)	60.6 % (263 of 434)	80.0 % (4 of 5)
Aromatic	53.7 % (36 of 67)	54.5 % (36 of 66)	0.0 % (0 of 1)
Methyl	78.4 % (40 of 51)	78.4 % (40 of 51)

1. Sso AcP

GSMKKWSDTE VFEMLKRMYA RVYGLVQGVG FRKFVQIHAI RLGIKGYAKN LPDGSVEVVA EGYEEALSKL LERIKQGPPA AEVEKVDYSF SEYKGEFEDF ETY

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Sso AcP			0.4 mM
2	sodium phosphate buffer			50 mM
3	NaCl			50 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1Y9O, Strand ID: A Detail

---

Coupling constant

60 J values in 1 lists
Field strength (¹H) 500 MHz, Temperature 310 (±0.1) K, pH 5.6 (±0.1) Detail

---

Temperature 310 (±0.1) K, pH 5.6 (±0.1)

List #1 J_coupling_list_1, Field strength (¹H) 500 MHz

Release date

2005-12-04

Citation

Structure, conformational stability, and enzymatic properties of acylphosphatase from the hyperthermophile Sulfolobus solfataricus
Corazza, A., Rosano, C., Pagano, K., Alverdi, V., Esposito, G., Capanni, C., Bemporad, F., Plakoutsi, G., Stefani, M., Chiti, F., Zuccotti, S., Bolognesi, M., Viglino, P.
Proteins (2006), 62, 64-79, PubMed 16287076 , DOI 10.1002/prot.20703 , Abstract

Related entities 1. Sulfolobus solfataricus acylphosphatase, : 1 : 8 : 89 entities Detail

Interaction partners 1. Sulfolobus solfataricus acylphosphatase, : 1 interactors Detail

Experiments performed 5 experiments Detail