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BMRB: 6401

2F77

NMR assignment of R55F mutant of Mason-Pfizer monkey virus matrix protein

Authors

Veverka, V., Vlach, J., Lipov, J., Rumlova, M., Ruml, T., Hrabal, R.

Assembly

R55F

Entity

1. R55F (polymer, Thiol state: all free), 100 monomers, 11959.62 Da Detail

MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT IDIKFWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM

Formula weight

11959.62 Da

Source organism

Mason-Pfizer monkey virus

Exptl. method

Refine. method

simulated annealing; molecular dynamics; torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.2 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.2 % (881 of 1274)	72.6 % (486 of 669)	61.2 % (304 of 497)	84.3 % (91 of 108)
Backbone	82.5 % (487 of 590)	98.5 % (197 of 200)	67.8 % (200 of 295)	94.7 % (90 of 95)
Sidechain	62.8 % (489 of 779)	61.6 % (289 of 469)	67.0 % (199 of 297)	7.7 % (1 of 13)
Aromatic	16.2 % (26 of 160)	32.5 % (26 of 80)	0.0 % (0 of 77)	0.0 % (0 of 3)
Methyl	83.3 % (80 of 96)	81.3 % (39 of 48)	85.4 % (41 of 48)

1. R55F

MGQELSQHER YVEQLKQALK TRGVKVKYAD LLKFFDFVKD TCPWFPQEGT IDIKFWRRVG DCFQDYYNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; HA

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 18 models in PDB: 2F77, Strand ID: X Detail

Release date

2005-07-25

Citation

Assignment of 1H, 13C, and 15N resonances of WT matrix protein and its R55F mutant from Mason-Pfizer monkey virus
Vlach, J., Lipov, J., Veverka, V., Rumlova, M., Ruml, T., Hrabal, R.
J. Biomol. NMR (2005), 31, 381-382, PubMed 15929014 , DOI 10.1007/s10858-005-2473-x , Abstract

Related entities 1. R55F, : 1 : 6 : 31 entities Detail

Interaction partners 1. R55F, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 1H15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

2 1H13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

3 HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

4 HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

5 HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

6 CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

7 CC-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

8 HcCH-COSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

9 3D 15N-edited NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

10 3D 13C-edited NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

11 4D 13C,15N-edited NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	R55F	[U-13C; U-15N]		1 mM
2	NaH2PO4/Na2HPO4			100 mM
3	NaCl			200 mM
4	D2O			10 %
5	DTT			5 %

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