BMRB: 6402

Solution structure of the carbon storage regulator CsrA from E. coli

Authors

Gutierrez, P.

Assembly

CsrA dimer

Entity

1. CsrA dimer (polymer, Thiol state: not present), 61 monomers, 6855.807 × 2 Da Detail

MLILTRRVGE TLMIGDEVTV TVLGVKGNQV RIGVNAPKEV SVHREEIYQR IQAEKSQQSS Y

Total weight

13711.614 Da

Max. entity weight

6855.807 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 59.2 %, Completeness (bb): 75.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	59.2 % (381 of 644)	56.6 % (210 of 371)	62.6 % (171 of 273)
Backbone	75.7 % (230 of 304)	87.3 % (110 of 126)	67.4 % (120 of 178)
Sidechain	52.3 % (207 of 396)	40.8 % (100 of 245)	70.9 % (107 of 151)
Aromatic	0.0 % (0 of 20)	0.0 % (0 of 10)	0.0 % (0 of 10)
Methyl	88.1 % (74 of 84)	88.1 % (37 of 42)	88.1 % (37 of 42)

1. Carbon storage regulator

MLILTRRVGE TLMIGDEVTV TVLGVKGNQV RIGVNAPKEV SVHREEIYQR IQAEKSQQSS Y

Show sample condition

Sample

Temperature 310 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Carbon storage regulator			1.0 ~ 4.0 mM

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LACS Plot; CA

Referencing offset: 2.83 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: 2.83 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Y00, Strand ID: A, B Detail

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Release date

2005-05-22

Citation

Solution structure of the carbon storage regulator protein CsrA from Escherichia coli
Gutierrez, P., Li, Y., Osborne, M.J., Pomerantseva, E., Liu, Q., Gehring, K.
J. Bacteriol. (2005), 187, 3496-3501, PubMed 15866937 , DOI 10.1128/JB.187.10.3496-3501.2005 , Abstract

Related entities 1. CsrA dimer, : 1 : 3 : 1 : 9 : 101 entities Detail

Interaction partners 1. CsrA dimer, : 12 interactors Detail

Experiments performed 1 experiments Detail