BMRB: 6403

Ovine Prion Protein Variant R168

Authors

Christen, B., Wuethrich, K.

Assembly

Ovine Prion Protein Variant R168

Entity

1. Ovine Prion Protein Variant R168 (polymer, Thiol state: all disulfide bound), 113 monomers, 13245.59 Da Detail

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDR YSNQNNFVHD CVNITVKQHT VTTTTKGENF TETDIKIMER VVEQMCITQY QRESQAYYQR GAS

Formula weight

13245.59 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Ovis aries

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.5 %, Completeness (bb): 82.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.5 % (1162 of 1328)	98.0 % (678 of 692)	71.2 % (363 of 510)	96.0 % (121 of 126)
Backbone	82.4 % (554 of 672)	97.8 % (227 of 232)	67.3 % (222 of 330)	95.5 % (105 of 110)
Sidechain	93.0 % (707 of 760)	98.0 % (451 of 460)	84.5 % (240 of 284)	100.0 % (16 of 16)
Aromatic	68.1 % (94 of 138)	89.9 % (62 of 69)	46.4 % (32 of 69)
Methyl	97.9 % (94 of 96)	97.9 % (47 of 48)	97.9 % (47 of 48)

1. sheep prion protein

GSVVGGLGGY MLGSAMSRPL IHFGNDYEDR YYRENMYRYP NQVYYRPVDR YSNQNNFVHD CVNITVKQHT VTTTTKGENF TETDIKIMER VVEQMCITQY QRESQAYYQR GAS

Show sample condition

Sample #1

Temperature 293 (±0.1) K, pH 4.50 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	sheep prion protein	[U-15N]		0.75 mM
2	sodium acetate	[U-2H]		10 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Temperature 293 (±0.1) K, pH 4.50 (±0.02)

#	Name	Isotope labeling	Type	Concentration
5	sheep prion protein	[U-13C; U-15N]		0.5 mM
6	sodium acetate	[U-2H]		10 mM
7	D2O			100 %

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LACS Plot; CA

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Y2S, Strand ID: A Detail

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Release date

2004-11-28

Citation

Prion protein NMR structures of cats, dogs, pigs, and sheep
Lysek, D.A., Schorn, C., Nivon, L.G., Esteve-Moya, V., Christen, B., Calzolai, L., von Schoetter, C., Fiorito, F., Herrmann, T., Guntert, P., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 640-645, PubMed 15647367 , DOI 10.1073/pnas.0408937102 , Abstract

Related entities 1. Ovine Prion Protein Variant R168, : 1 : 8 : 2 : 152 entities Detail

Interaction partners 1. Ovine Prion Protein Variant R168, : 1 interactors Detail

Experiments performed 3 experiments Detail