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BMRB: 6410

1Z7R

1H, 15N, and 13C resonance assignments of reduced form of poplar glutaredoxin CxxC1 from Populus tremula tremuloides

Authors

Feng, Y., Rouhier, N., Jacquot, J., Xia, B.

Assembly

grx1

Entity

1. grx1 (polymer, Thiol state: all free), 117 monomers, 12514.18 Da Detail

MASKQELDAA LKKAKELASS APVVVFSKTY CGYCNRVKQL LTQVGASYKV VELDELSDGS QLQSALAHWT GRGTVPNVFI GGKQIGGCDT VVEKHQRNEL LPLLQDAAAT AKNPAQL

Formula weight

12514.18 Da

Source organism

Populus tremula x Populus tremuloides

Exptl. method

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 97.5 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.5 % (1286 of 1319)	97.7 % (666 of 682)	97.5 % (497 of 510)	96.9 % (123 of 127)
Backbone	96.7 % (671 of 694)	96.7 % (231 of 239)	96.8 % (331 of 342)	96.5 % (109 of 113)
Sidechain	98.1 % (719 of 733)	98.2 % (435 of 443)	97.8 % (270 of 276)	100.0 % (14 of 14)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (31 of 31)	100.0 % (1 of 1)
Methyl	98.6 % (146 of 148)	98.6 % (73 of 74)	98.6 % (73 of 74)

1. grxcxxc1

MASKQELDAA LKKAKELASS APVVVFSKTY CGYCNRVKQL LTQVGASYKV VELDELSDGS QLQSALAHWT GRGTVPNVFI GGKQIGGCDT VVEKHQRNEL LPLLQDAAAT AKNPAQL

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1Z7R, Strand ID: A Detail

Release date

2005-12-14

Citation

1H, 15N, and 13C resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides
Feng, Y., Rouhier, N., Jacquot, J., Xia, B.
J. Biomol. NMR (2005), 31, 263-264, PubMed 15803403 , DOI 10.1007/s10858-005-0497-x , Abstract

Related entities 1. grx1, : 1 : 1 : 1 : 199 entities Detail

Experiments performed 20 experiments Detail

1 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

2 HNCACB

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

3 CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

4 HNCA

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

5 HNCO

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

6 HN(CA)CO

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

7 HBHA(CBCA)(CO)NH

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

8 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

9 H(C)CH-TOCSY

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

10 (H)CCH-TOCSY

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

11 H(C)CH-COSY

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

12 (H)CCH-COSY

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

13 (HB)CB(CGCD)HD

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

14 (HB)CB(CGCDCE)HE

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

15 1H-13C NOESY-HSQC(optimized for aromatic region)

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

16 1H-15N TOCSY-HSQC

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

17 H(CCO)NH

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

18 (H)C(CO)NH

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

19 1H-15N NOESY-HSQC

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

20 1H-13C NOESY-HSQC

Instrument

Bruker Avance - 500 MHz Bruker Avance 500 MHz spectrometer with a triple resonance cryo-probe equipped with Z-axis gradient.

Sample

State isotropic, Temperature 293 (±0.1) K, pH 6.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	grxcxxc1	[U-15N; U-13C]		1.1 mM
2	DTT			40 mM
3	DSS			0.01 %
4	NaN3			0.01 %
5	PBS			40 mM

nullKeywords Glutaredoxin, Poplar