Search

Query history

Search help 
Download

Found 1 Document (1.805 seconds)

BMRB: 6416


6416
1UIV
Backbone 1H, 13C, and 15N assignments of a 56 kDa E. coli nickel binding protein NikA
Authors
Rajesh, S., Heddle, J.G., Kurashima-Ito, K., Nietlispach, D., Shirakawa, M., Tame, J.R.H., Ito, Y.
Assembly
apo-NikA
Entity
1. apo-NikA (polymer, Thiol state: not present), 502 monomers, 56329.35 Da Detail

AAPDEITTAW PVNVGPLNPH LYTPNQMFAQ SMVYEPLVKY QADGSVIPWL AKSWTHSEDG KTWTFTLRDD VKFSNGEPFD AEAAAENFRA VLDNRQRHAW LELANQIVDV KALSKTELQI TLKSAYYPFL QELALPRPFR FIAPSQFKNH ETMNGIKAPI GTGPWILQES KLNQYDVFVR NENYWGEKPA IKKITFNVIP DPTTRAVAFE TGDIDLLYGN EGLLPLDTFA RFSQNPAYHT QLSQPIETVM LALNTAKAPT NELAVREALN YAVNKKSLID NALYGTQQVA DTLFAPSVPY ANLGLKPSQY DPQKAKALLE KAGWTLPAGK DIREKNGQPL RIELSFIGTD ALSKSMAEII RADMRQIGAD VSLIGEEESS IYARQRDGRF GMIFHRTWGA PYDPHAFLSS MRVPSHADFQ AQQGLADKPL IDKEIGEVLA THDETQRQAL YRDILTRLHD EAVYLPISYI SMMVVSKPEL GNIPYAPIAT EIPFEQIKPV KP


Formula weight
56329.35 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.4 %, Completeness: 46.4 %, Completeness (bb): 77.2 % Detail
Download

Polymer type: polypeptide(L)

Total1H13C15N
All46.4 % (2752 of 5931)29.6 % (913 of 3084)60.8 % (1414 of 2325)81.4 % (425 of 522)
Backbone77.2 % (2267 of 2936)54.2 % (537 of 991)88.7 % (1314 of 1481)89.7 % (416 of 464)
Sidechain26.1 % (905 of 3472)18.0 % (377 of 2093)39.3 % (519 of 1321)15.5 % (9 of 58)
Aromatic 8.2 % (42 of 510)10.2 % (26 of 255) 6.1 % (15 of 246)11.1 % (1 of 9)
Methyl22.1 % (128 of 578)19.0 % (55 of 289)25.3 % (73 of 289)

1. periplasmic nickel binding protein A

AAPDEITTAW PVNVGPLNPH LYTPNQMFAQ SMVYEPLVKY QADGSVIPWL AKSWTHSEDG KTWTFTLRDD VKFSNGEPFD AEAAAENFRA VLDNRQRHAW LELANQIVDV KALSKTELQI TLKSAYYPFL QELALPRPFR FIAPSQFKNH ETMNGIKAPI GTGPWILQES KLNQYDVFVR NENYWGEKPA IKKITFNVIP DPTTRAVAFE TGDIDLLYGN EGLLPLDTFA RFSQNPAYHT QLSQPIETVM LALNTAKAPT NELAVREALN YAVNKKSLID NALYGTQQVA DTLFAPSVPY ANLGLKPSQY DPQKAKALLE KAGWTLPAGK DIREKNGQPL RIELSFIGTD ALSKSMAEII RADMRQIGAD VSLIGEEESS IYARQRDGRF GMIFHRTWGA PYDPHAFLSS MRVPSHADFQ AQQGLADKPL IDKEIGEVLA THDETQRQAL YRDILTRLHD EAVYLPISYI SMMVVSKPEL GNIPYAPIAT EIPFEQIKPV KP

Show sample condition
Sample

Temperature 303 (±1) K, pH 7.5 (±0.2)


#NameIsotope labelingTypeConcentration
1periplasmic nickel binding protein A[U-99% 2H; U-13C; U-15N]1.5 ~ 2.0 mM

LACS Plot; CA
Referencing offset: 0.91 ppm, Outliers: 6 Detail
LACS Plot; CB
Referencing offset: 0.91 ppm, Outliers: 6 Detail
LACS Plot; CO
Referencing offset: 0.45 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1UIV, Strand ID: A, B Detail

Release date
2005-08-15
Citation 1
Backbone 1H, 13C, and 15N assignments of a 56 kDa E. coli nickel binding protein NikA
Rajesh, S., Heddle, J.G., Kurashima-Ito, K., Nietlispach, D., Shirakawa, M., Tame, J.R.H., Ito, Y.
J. Biomol. NMR (2005), 32, 177-177, PubMed 16034673 , DOI 10.1007/s10858-005-6072-7 ,
Show more 1 citaion
Citation 2
Crystal structures of the liganded and unliganded nickel-binding protein NikA from Escherichia coli
Heddle, J., Scott, D.J., Unzai, S., Park, S.Y., Tame, J.R.
J. Biol. Chem. (2003), 278, 50322-50329, PubMed 12960164 , DOI 10.1074/jbc.M307941200 ,
Related entities 1. apo-NikA, : 1 : 1 : 25 : 113 entities Detail
More details for 140 related entities
Interaction partners 1. apo-NikA, : 1 interactors Detail
More details for 1 interactors identified by 1 citations
Experiments performed 5 experiments Detail
nullKeywords Deuteration, TROSY, NMR assignments, nickel, NikA