BMRB: 6419

Structure of the C-terminal Domain from Trypanosoma brucei Variant Surface Glycoprotein MITat1.2

Authors

Chattopadhyay, A., Jones, N.G., Nietlispach, D., Nielsen, P.R., Voorheis, H.P., Mott, H.R., Carrington, M.

Assembly

Variant surface glycoprotein MITAT 1.2

Entity

1. Variant surface glycoprotein MITAT 1.2 (polymer, Thiol state: all disulfide bound), 80 monomers, 8734.426 Da Detail

GSHMLEVLTQ KHKPAESQQQ AAETEGSCNK KDQNECKSPC KWHNDAENKK CTLDKEEAKK VADETAKDGK TGNTNTTGSS

Formula weight

8734.426 Da

Entity Connection

disulfide 2 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS28:SG	1:CYS40:SG
2	disulfide	sing	1:CYS36:SG	1:CYS51:SG

Source organism

Trypanosoma brucei

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.5 %, Completeness (bb): 81.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.5 % (703 of 884)	82.1 % (384 of 468)	73.6 % (240 of 326)	87.8 % (79 of 90)
Backbone	81.7 % (389 of 476)	94.5 % (154 of 163)	68.9 % (162 of 235)	93.6 % (73 of 78)
Sidechain	80.1 % (387 of 483)	75.4 % (230 of 305)	91.0 % (151 of 166)	50.0 % (6 of 12)
Aromatic	8.3 % (2 of 24)	8.3 % (1 of 12)	0.0 % (0 of 11)	100.0 % (1 of 1)
Methyl	96.0 % (48 of 50)	96.0 % (24 of 25)	96.0 % (24 of 25)

1. Variant surface glycoprotein MITAT 1.2

GSHMLEVLTQ KHKPAESQQQ AAETEGSCNK KDQNECKSPC KWHNDAENKK CTLDKEEAKK VADETAKDGK TGNTNTTGSS

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Variant surface glycoprotein MITAT 1.2	[U-15N]		1 mM
2	sodium phosphate			50 mM
3	sodium chloride			100 mM
4	sodium azide			0.05 %
5	H2O			90 %
6	D2O			10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	Variant surface glycoprotein MITAT 1.2	[U-13C; U-15N]		1 mM
8	sodium phosphate			50 mM
9	sodium chloride			100 mM
10	sodium azide			0.05 %
11	H2O			90 %
12	D2O			10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 60 models in PDB: 1XU6, Strand ID: A Detail

---

Release date

2004-12-09

Citation

Structure of the C-terminal domain from Trypanosoma brucei variant surface glycoprotein MITat1.2
Chattopadhyay, A., Jones, N.G., Nietlispach, D., Nielsen, P.R., Voorheis, H.P., Mott, H.R., Carrington, M.
J. Biol. Chem. (2005), 280, 7228-7235, PubMed 15557330 , DOI 10.1074/jbc.M410787200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Variant surface glycoprotein MITAT 1.2, : 1 : 1 : 1 entities Detail

Experiments performed 3 experiments Detail