BMRB: 6434

Backbone and Sidechain Assignments of the Lambda Bacteriophage Tail Assembly Protein gpU

Authors

Edmonds, L., Thirumoorthy, R., Liu, A., Davidson, A., Donaldson, L.W.

Assembly

gpU

Entity

1. gpU (polymer, Thiol state: not present), 150 monomers, 16681.15 Da Detail

GSSHHHHHHS SGLVPRGSHM KHTELRAAVL DALEKHDTGA TFFDGRPAVF DEADFPAVAV YLTGAEYTGE ELDSDTWQAE LHIEVFLPAQ VPDSELDAWM ESRIYPVMSD IPALSDLITS MVASGYDYRR DDDAGLWSSA DLTYVITYEM

2. MG (non-polymer), 24.305 Da

Total weight

16705.455 Da

Max. entity weight

16681.15 Da

Source organism

Escherichia virus Lambda

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.7 %, Completeness: 84.0 %, Completeness (bb): 89.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.0 % (1388 of 1653)	83.7 % (701 of 838)	83.1 % (554 of 667)	89.9 % (133 of 148)
Backbone	89.4 % (792 of 886)	90.1 % (272 of 302)	88.9 % (392 of 441)	89.5 % (128 of 143)
Sidechain	79.8 % (725 of 908)	80.0 % (429 of 536)	79.3 % (291 of 367)	100.0 % (5 of 5)
Aromatic	50.0 % (91 of 182)	52.7 % (48 of 91)	45.5 % (40 of 88)	100.0 % (3 of 3)
Methyl	98.1 % (159 of 162)	98.8 % (80 of 81)	97.5 % (79 of 81)

1. gpU

GSSHHHHHHS SGLVPRGSHM KHTELRAAVL DALEKHDTGA TFFDGRPAVF DEADFPAVAV YLTGAEYTGE ELDSDTWQAE LHIEVFLPAQ VPDSELDAWM ESRIYPVMSD IPALSDLITS MVASGYDYRR DDDAGLWSSA DLTYVITYEM

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 7.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	gpU	[U-98% 13C; U-98% 15N]		0.8 mM
2	deutero-Tris	[U-d10]		5 mM
3	KCl			50 mM
4	sodium azide			0.02 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -2.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1Z1Z, Strand ID: A Detail

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Release date

2005-07-25

Citation

NMR assignment of the gpU tail protein from lambda bacteriophage
Edmonds, L., Thirumoorthy, R., Liu, A., Davidson, A., Donaldson, L.W.
J. Biomol. NMR (2005), 32, 91-92, PubMed 16041488 , DOI 10.1007/s10858-005-3659-y , Abstract

Related entities 1. gpU, : 1 : 8 : 7 entities Detail

Interaction partners 1. gpU, : 8 interactors Detail

Experiments performed 3 experiments Detail