BMRB: 6437

Structural origin of endotoxin neutralization and antimicrobial activity of a lactoferrin-based peptide

Authors

Japelj, B., Pristovsek, P., Majerle, A., Jerala, R.

Assembly

lf11 peptide

Entity

1. lf11 peptide (polymer, Thiol state: not present), 11 monomers, 1530.776 Da Detail

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Formula weight

1530.776 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 98.9 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.9 % (89 of 90)	98.9 % (89 of 90)
Backbone	95.5 % (21 of 22)	95.5 % (21 of 22)
Sidechain	100.0 % (68 of 68)	100.0 % (68 of 68)
Aromatic	100.0 % (11 of 11)	100.0 % (11 of 11)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. lf11 peptide

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Show sample condition

Sample

Temperature 303 (±0.05) K, pH 5.5 (±0.1), Details LPS (Lipopolysaccharide) of Escherichia coli,serotype 055:B5.

#	Name	Isotope labeling	Type	Concentration
1	lf11 peptide			2 mM
2	sodium phosphate			20 mM
3	D2O			8 %
4	LPS			1 mg/mL

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Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 90.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.2 % (175 of 180)	97.2 % (175 of 180)
Backbone	90.9 % (40 of 44)	90.9 % (40 of 44)
Sidechain	99.3 % (135 of 136)	99.3 % (135 of 136)
Aromatic	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. lf11 peptide

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Sample

Temperature 303 (±0.05) K, pH 5.5 (±0.1), Details SDS-d25 (fully deuterated sodium dodecyl sulphate)

#	Name	Isotope labeling	Type	Concentration
5	lf11 peptide			2 mM
6	SDS-d25			200 mM
7	sodium phosphate			20 mM
8	D2O			8 %

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Chem. Shift Complete3

Sequence coverage: 100.0 %, Completeness: 97.4 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.4 % (263 of 270)	97.4 % (263 of 270)
Backbone	92.4 % (61 of 66)	92.4 % (61 of 66)
Sidechain	99.0 % (202 of 204)	99.0 % (202 of 204)
Aromatic	97.0 % (32 of 33)	97.0 % (32 of 33)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. lf11 peptide

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Show sample condition

Sample

Temperature 303 (±0.05) K, pH 5.5 (±0.1), Details DPC-d38 (fully deuterated dodecylphosphocholine)

#	Name	Isotope labeling	Type	Concentration
9	lf11 peptide			2 mM
10	DPC-d38			240 mM
11	sodium phosphate			20 mM
12	D2O			8 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2GMD, Strand ID: A Detail

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Release date

2005-11-13

Citation

Structural origin of endotoxin neutralization and antimicrobial activity of a lactoferrin-based peptide
Japelj, B.T., Pristovsek, P., Majerle, A., Jerala, R.
J. Biol. Chem. (2005), 280, 16955-16961, PubMed 15687491 , DOI 10.1074/jbc.M500266200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. lf11 peptide, : 1 : 4 : 26 : 2 entities Detail

Interaction partners 1. lf11 peptide, : 19 interactors Detail

Experiments performed 2 experiments Detail