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BMRB: 6438

2J48

Sequence-specific resonance assignments of the C-terminal, 137-residue pseudo-receiver domain of circadian input kinase (CikA) that resets the circadian clock in Synechococcus elongatus

Authors

Gao, T., Zhang, X., Xia, Y., Golden, S.S., LiWang, A.C.

Assembly

CikA C-terminus

Entity

1. CikA C-terminus (polymer, Thiol state: all free), 137 monomers, 14771.96 Da Detail

GSHMIDNLPA GHILLLEEED EAATVVCEML TAAGFKVIWL VDGSTALDQL DLLQPIVILM AWPPPDQSCL LLLQHLREHQ ADPHPPLVLF LGEPPVDPLL TAQASAILSK PLDPQLLLTT LQGLCPPNLS EGDRPSS

Formula weight

14771.96 Da

Source organism

Synechococcus elongatus

Exptl. method

NMR

Refine. method

XPLOR-NIH

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 88.9 %, Completeness (bb): 82.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.9 % (1388 of 1561)	95.2 % (766 of 805)	79.1 % (493 of 623)	97.0 % (129 of 133)
Backbone	82.0 % (648 of 790)	94.0 % (249 of 265)	69.3 % (280 of 404)	98.3 % (119 of 121)
Sidechain	95.0 % (856 of 901)	94.4 % (510 of 540)	96.3 % (336 of 349)	83.3 % (10 of 12)
Aromatic	71.9 % (46 of 64)	71.9 % (23 of 32)	76.7 % (23 of 30)	0.0 % (0 of 2)
Methyl	95.5 % (193 of 202)	93.1 % (94 of 101)	98.0 % (99 of 101)

1. CikA

GSHMIDNLPA GHILLLEEED EAATVVCEML TAAGFKVIWL VDGSTALDQL DLLQPIVILM AWPPPDQSCL LLLQHLREHQ ADPHPPLVLF LGEPPVDPLL TAQASAILSK PLDPQLLLTT LQGLCPPNLS EGDRPSS

Show sample condition

Sample

Temperature 298 (±0.05) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	CikA	[U-99% 13C; U-99% 15N]	1.3 mM
2	NaCL		50 mM
3	Phosphate		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2J48, Strand ID: A Detail

Release date

2005-08-28

Citation

1H, 13C and 15N Chemical Shift Assignments of the C-Terminal, 133-Residue Pseudo-Receiver Domain of Circadian Input Kinase (CikA) in Synechococcus elongatus
Gao, T., Zhang, X., Xia, Y., Cho, Y., Sacchettini, J.C., Golden, S.S., LiWang, A.C.
J. Biomol. NMR (2005), 32, 259-259, PubMed , DOI:

Related entities 1. CikA C-terminus, : 1 : 79 entities Detail

Experiments performed 14 experiments Detail

1 15N HSQC