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BMRB: 6443

1YDU

Assignments of At5g01610, an Arabidopsis thaliana protein containign DUF583 domain

Authors

Zhao, Q., Cornilescu, C., Lee, M., Markley, J.

Assembly

At5g01610

Entity

1. At5g01610 (polymer, Thiol state: all free), 170 monomers, 18952.51 Da Detail

SDQIFNKVGS YWLGQKANKQ FDSVGNDLNS VSTSIEGGTK WLVNKIKGKM QKPLPELLKE YDLPIGIFPG DATNYEFDEE TKKLTVLIPS ICEVGYKDSS VLKFTTTVTG HLEKGKLTDV EGIKTKVMIW VKVTSISTDA SKVYFTAGMK KSRSRDAYGV QRNGLRVDKF

Formula weight

18952.51 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

water refinement with XPLOR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 74.1 %, Completeness: 63.5 %, Completeness (bb): 71.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	63.5 % (1282 of 2019)	63.7 % (669 of 1051)	62.3 % (491 of 788)	67.8 % (122 of 180)
Backbone	71.1 % (718 of 1010)	71.4 % (250 of 350)	70.3 % (348 of 495)	72.7 % (120 of 165)
Sidechain	58.4 % (680 of 1164)	59.9 % (420 of 701)	57.6 % (258 of 448)	13.3 % (2 of 15)
Aromatic	0.6 % (1 of 158)	1.3 % (1 of 79)	0.0 % (0 of 76)	0.0 % (0 of 3)
Methyl	74.2 % (141 of 190)	77.9 % (74 of 95)	70.5 % (67 of 95)

1. At5g01610

SDQIFNKVGS YWLGQKANKQ FDSVGNDLNS VSTSIEGGTK WLVNKIKGKM QKPLPELLKE YDLPIGIFPG DATNYEFDEE TKKLTVLIPS ICEVGYKDSS VLKFTTTVTG HLEKGKLTDV EGIKTKVMIW VKVTSISTDA SKVYFTAGMK KSRSRDAYGV QRNGLRVDKF

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 5.18 (±0.02)

#	Name	Isotope labeling	Concentration
1	At5g01610	[U-95% 13C; U-90% 15N]	10 mg/mL
2	NaPi		20 mM
3	NaCl		25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	methyl protons	4.77 ppm	internal	direct	1.0
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	methyl protons	4.77 ppm	internal	direct	1.0
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	methyl protons	4.77 ppm	internal	direct	1.0
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	methyl protons	4.77 ppm	internal	direct	1.0
¹³C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

Referencing offset: 0.61 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1YDU, Strand ID: A Detail

Release date

2005-04-14

Citation

Solution NMR structure of At5g01610, an Arabdopsis protein containing DUF538 domain
Zhao, Q., Cornilescu, C., Lee, M.S., Markley, J.L.

Related entities 1. At5g01610, : 1 : 2 : 18 entities Detail

Experiments performed 14 experiments Detail

1 HNCA