BMRB: 6446

Structural and Functional Characterization of Transmembrane Segment IV of the NHE1 Isoform of the Na+/H+ Exchanger

Authors

Slepkov, E.R., Rainey, J.K., Li, X., Liu, Y., Cheng, F.J., Lindhout, D.A., Sykes, B.D., Fliegel, L.

Assembly

Sodium/hydrogen exchanger 1

Entity

1. Sodium/hydrogen exchanger 1 (polymer, Thiol state: not present), 27 monomers, 3138.738 Da Detail

FLQSDVFFLF LLPPIILDAG YFLPLRX

Formula weight

3138.738 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 59.7 %, Completeness (bb): 81.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	59.7 % (206 of 345)	72.9 % (129 of 177)	37.5 % (54 of 144)	95.8 % (23 of 24)
Backbone	81.3 % (122 of 150)	98.0 % (49 of 50)	66.2 % (51 of 77)	95.7 % (22 of 23)
Sidechain	49.5 % (109 of 220)	63.0 % (80 of 127)	30.4 % (28 of 92)	100.0 % (1 of 1)
Aromatic	0.0 % (0 of 58)	0.0 % (0 of 29)	0.0 % (0 of 29)
Methyl	40.5 % (17 of 42)	71.4 % (15 of 21)	9.5 % (2 of 21)

1. Sodium/hydrogen exchanger 1

FLQSDVFFLF LLPPIILDAG YFLPLRX

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, Details CDC13/CD3OH/H2O ratio was actually 4:4:1 V:v:v

#	Name	Isotope labeling	Type	Concentration
1	Sodium/hydrogen exchanger 1			2 mM
2	DSS			1 mM
3	CDCl3			44 %
4	CD3OH			44 %
5	H2O			11 %

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Calculated from 100 models in PDB: 1Y4E, Strand ID: A Detail

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Release date

2005-06-01

Citation

Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger
Slepkov, E.R., Rainey, J.K., Li, X., Liu, Y., Cheng, F.J., Lindhout, D.A., Sykes, B.D., Fliegel, L.
J. Biol. Chem. (2005), 280, 17863-17872, PubMed 15677483 , DOI 10.1074/jbc.M409608200 , Abstract

Related entities 1. Sodium/hydrogen exchanger 1, : 1 : 7 : 65 entities Detail

Interaction partners 1. Sodium/hydrogen exchanger 1, : 13 interactors Detail

Experiments performed 4 experiments Detail