BMRB: 6447

Solution structure of a two-repeat fragment of major vault protein

Authors

Kozlov, G., Vavelyuk, O., Minailiuc, O., Banville, D., Gehring, K., Ekiel, I.

Assembly

Major vault protein

Entity

1. Major vault protein (polymer, Thiol state: not present), 113 monomers, 12757.43 Da Detail

GSHMQVVLPN TALHLKALLD FEDKDGDKVV AGDEWLFEGP GTYIPRKEVE VVEIIQATII RQNQALRLRA RKECWDRDGK ERVTGEEWLV TTVGAYLPAV FEEVLDLVDA VIL

Formula weight

12757.43 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 65.9 %, Completeness (bb): 80.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	65.9 % (871 of 1321)	77.4 % (527 of 681)	44.8 % (234 of 522)	93.2 % (110 of 118)
Backbone	80.1 % (537 of 670)	93.9 % (216 of 230)	65.0 % (215 of 331)	97.2 % (106 of 109)
Sidechain	57.0 % (431 of 756)	69.0 % (311 of 451)	39.2 % (116 of 296)	44.4 % (4 of 9)
Aromatic	38.9 % (35 of 90)	71.1 % (32 of 45)	0.0 % (0 of 42)	100.0 % (3 of 3)
Methyl	57.4 % (93 of 162)	88.9 % (72 of 81)	25.9 % (21 of 81)

1. Major vault protein

GSHMQVVLPN TALHLKALLD FEDKDGDKVV AGDEWLFEGP GTYIPRKEVE VVEIIQATII RQNQALRLRA RKECWDRDGK ERVTGEEWLV TTVGAYLPAV FEEVLDLVDA VIL

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Major vault protein	[U-15N]		1 mM
2	bis-Tris			20 mM
3	NaCl			150 mM
4	CaCl2			2 mM
5	DTT			15 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.76 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.76 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1Y7X, Strand ID: A Detail

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Release date

2007-01-28

Citation

Solution structure of a two-repeat fragment of major vault protein
Kozlov, G., Vavelyuk, O., Minailiuc, O., Banville, D., Gehring, K., Ekiel, I.
J. Mol. Biol. (2006), 356, 444-452, PubMed 16373071 , DOI 10.1016/j.jmb.2005.11.064 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Major vault protein, : 1 : 69 entities Detail

Interaction partners 1. Major vault protein, : 35 interactors Detail

Experiments performed 3 experiments Detail